The relative availability of inorganic carbon and inorganic nitrogen influences the response of the dinoflagellate Protoceratium reticulatum to elevated CO2

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388971%3A_____%2F17%3A00474561" target="_blank" >RIV/61388971:_____/17:00474561 - isvavai.cz</a>

Výsledek na webu

<a href="http://dx.doi.org/10.1111/jpy.12463" target="_blank" >http://dx.doi.org/10.1111/jpy.12463</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1111/jpy.12463" target="_blank" >10.1111/jpy.12463</a>

Jazyk výsledku

angličtina

Název v původním jazyce

The relative availability of inorganic carbon and inorganic nitrogen influences the response of the dinoflagellate Protoceratium reticulatum to elevated CO2

Popis výsledku v původním jazyce

This work originates from three facts: (i) changes in CO2 availability influence metabolic processes in algal cells, (ii) Spatial and temporal variations of nitrogen availability cause repercussions on phytoplankton physiology, (iii) Growth and cell composition are dependent on the stoichiometry of nutritional resources. In this study, we assess whether the impact of rising pCO(2) is influenced by N availability, through the impact that it would have on the C/N stoichiometry, in conditions of N sufficiency. Our experiments used the dinoflagellate Protoceratium reticulatum, which we cultured under three CO2 regimes (400, 1,000, and 5,000 ppmv, pH of 8.1) and either variable (the NO3- concentration was always 2.5 mmol.L-1) or constant (NO3- concentration varied to maintain the same Ci/NO3- ratio at all pCO(2)) C-i/NO3- ratio. Regardless of N availability, cells had higher specific growth rates, but lower cell dry weight and C and N quotas, at elevated CO2. The carbohydrate pool size and the C/N was unaltered in all treatments. The lipid content only decreased at high pCO(2) at constant Ci/NO3- ratio. In the variable Ci/NO3- conditions, the relative abundance of Rubisco (and other proteins) also changed, this did not occur at constant Ci/NO3-. Thus, the biomass quality of P. reticulatum for grazers was affected by the Ci/NO3- ratio in the environment and not only by the pCO(2), both with respect to the size of the main organic pools and the composition of the expressed proteome.

Název v anglickém jazyce

The relative availability of inorganic carbon and inorganic nitrogen influences the response of the dinoflagellate Protoceratium reticulatum to elevated CO2

Popis výsledku anglicky

This work originates from three facts: (i) changes in CO2 availability influence metabolic processes in algal cells, (ii) Spatial and temporal variations of nitrogen availability cause repercussions on phytoplankton physiology, (iii) Growth and cell composition are dependent on the stoichiometry of nutritional resources. In this study, we assess whether the impact of rising pCO(2) is influenced by N availability, through the impact that it would have on the C/N stoichiometry, in conditions of N sufficiency. Our experiments used the dinoflagellate Protoceratium reticulatum, which we cultured under three CO2 regimes (400, 1,000, and 5,000 ppmv, pH of 8.1) and either variable (the NO3- concentration was always 2.5 mmol.L-1) or constant (NO3- concentration varied to maintain the same Ci/NO3- ratio at all pCO(2)) C-i/NO3- ratio. Regardless of N availability, cells had higher specific growth rates, but lower cell dry weight and C and N quotas, at elevated CO2. The carbohydrate pool size and the C/N was unaltered in all treatments. The lipid content only decreased at high pCO(2) at constant Ci/NO3- ratio. In the variable Ci/NO3- conditions, the relative abundance of Rubisco (and other proteins) also changed, this did not occur at constant Ci/NO3-. Thus, the biomass quality of P. reticulatum for grazers was affected by the Ci/NO3- ratio in the environment and not only by the pCO(2), both with respect to the size of the main organic pools and the composition of the expressed proteome.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10606 - Microbiology

Projekt

—

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2017

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Journal of Phycology

ISSN

0022-3646

e-ISSN

—

Svazek periodika

53

Číslo periodika v rámci svazku

2

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

10

Strana od-do

298-307

Kód UT WoS článku

000399745800006

EID výsledku v databázi Scopus

2-s2.0-84999016807