Flavonolignan 2,3-dehydrosilydianin activates Nrf2 and upregulates NAD(P)H:quinone oxidoreductase 1 in Hepa1c1c7 cells

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388971%3A_____%2F17%3A00476118" target="_blank" >RIV/61388971:_____/17:00476118 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/61989592:15110/17:73581541

Výsledek na webu

<a href="http://dx.doi.org/10.1016/j.fitote.2017.04.012" target="_blank" >http://dx.doi.org/10.1016/j.fitote.2017.04.012</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.fitote.2017.04.012" target="_blank" >10.1016/j.fitote.2017.04.012</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Flavonolignan 2,3-dehydrosilydianin activates Nrf2 and upregulates NAD(P)H:quinone oxidoreductase 1 in Hepa1c1c7 cells

Popis výsledku v původním jazyce

This study examined whether the flavonolignans (silybin, silychristin, silydianin, 2,3-dehydrosilybin, 2,3-dehydrosilychristin, 2,3-dehydrosilydianin) activate the Nrf2 pathway, which regulates the expression of many cytoprotective enzymes, including NAD(P)H:quinone oxidoreductase 1 (NQO1). We found that 2,3-dehydrosilydianin significantly elevated activity of NQO1 in Hepa1c1c7 cells. In contrast, other tested compounds had mild or negligible effect on NQO1 activity. We also found that 2,3-dehydrosilydianin activated transcription via antioxidant response element in AREc32 reporter cells, caused the accumulation of Nrf2 and induced the expression of Nqo1 gene at mRNA and protein levels in Hepa1c1c7 cells. 2,3-Dehydrosilydianin also increased the expression of heme oxygenase-1 gene and glutamate-cysteine ligase modifier subunit gene. We conclude that 2,3-dehydrosilydianin activates Nrf2 and induces Nrf2-mediated gene expression in Hepa1c1c7 cells

Název v anglickém jazyce

Flavonolignan 2,3-dehydrosilydianin activates Nrf2 and upregulates NAD(P)H:quinone oxidoreductase 1 in Hepa1c1c7 cells

Popis výsledku anglicky

This study examined whether the flavonolignans (silybin, silychristin, silydianin, 2,3-dehydrosilybin, 2,3-dehydrosilychristin, 2,3-dehydrosilydianin) activate the Nrf2 pathway, which regulates the expression of many cytoprotective enzymes, including NAD(P)H:quinone oxidoreductase 1 (NQO1). We found that 2,3-dehydrosilydianin significantly elevated activity of NQO1 in Hepa1c1c7 cells. In contrast, other tested compounds had mild or negligible effect on NQO1 activity. We also found that 2,3-dehydrosilydianin activated transcription via antioxidant response element in AREc32 reporter cells, caused the accumulation of Nrf2 and induced the expression of Nqo1 gene at mRNA and protein levels in Hepa1c1c7 cells. 2,3-Dehydrosilydianin also increased the expression of heme oxygenase-1 gene and glutamate-cysteine ligase modifier subunit gene. We conclude that 2,3-dehydrosilydianin activates Nrf2 and induces Nrf2-mediated gene expression in Hepa1c1c7 cells

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10608 - Biochemistry and molecular biology

Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2017

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Fitoterapia

ISSN

0367-326X

e-ISSN

—

Svazek periodika

119

Číslo periodika v rámci svazku

Jun 2017

Stát vydavatele periodika

IT - Italská republika

Počet stran výsledku

6

Strana od-do

115-120

Kód UT WoS článku

000404307800018

EID výsledku v databázi Scopus

2-s2.0-85018372648