Disseminated infection due to Exophiala pisciphila in Cardinal tetra, Paracheirodon axelrodi

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388971%3A_____%2F17%3A00476499" target="_blank" >RIV/61388971:_____/17:00476499 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/00100595:_____/17:N0000017 RIV/00216208:11310/17:10371451

Výsledek na webu

<a href="http://dx.doi.org/10.1111/jfd.12577" target="_blank" >http://dx.doi.org/10.1111/jfd.12577</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1111/jfd.12577" target="_blank" >10.1111/jfd.12577</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Disseminated infection due to Exophiala pisciphila in Cardinal tetra, Paracheirodon axelrodi

Popis výsledku v původním jazyce

Cardinal tetra, Paracheirodon axelrodi (Schultz, 1956), kept in an ornamental tank, was found to be affected by severe invasive mycosis. Externally, the disease manifested as abdominal swelling, and internally, the anterior part of the intestine was extremely bloated with abundant dematiaceous septate hyphae and an accumulation of fluid. Histopathologically, a granulomatous inflammatory response was observed in the intestine wall, kidney and spleen. We assume that the mycotic agent was primarily deposited in the intestine and was then gradually disseminated to the other organs. DNA sequencing of ITS and LSU rDNA regions and phenotypic characterization were used for identification of the isolated fungus. The obtained data confirmed that the infection was caused by Exophiala pisciphila. The disease was subsequently reproduced in the carp fingerling using intramuscular and intraperitoneal injection of a spore suspension. The 13th day after intramuscular infection, a marked elevation of neutrophils was recorded in the peripheral blood, this involved a proliferation of band forms and segmented forms. As far as we know, this is the first report of infection due to E.pisciphila in Cardinal tetra.

Název v anglickém jazyce

Disseminated infection due to Exophiala pisciphila in Cardinal tetra, Paracheirodon axelrodi

Popis výsledku anglicky

Cardinal tetra, Paracheirodon axelrodi (Schultz, 1956), kept in an ornamental tank, was found to be affected by severe invasive mycosis. Externally, the disease manifested as abdominal swelling, and internally, the anterior part of the intestine was extremely bloated with abundant dematiaceous septate hyphae and an accumulation of fluid. Histopathologically, a granulomatous inflammatory response was observed in the intestine wall, kidney and spleen. We assume that the mycotic agent was primarily deposited in the intestine and was then gradually disseminated to the other organs. DNA sequencing of ITS and LSU rDNA regions and phenotypic characterization were used for identification of the isolated fungus. The obtained data confirmed that the infection was caused by Exophiala pisciphila. The disease was subsequently reproduced in the carp fingerling using intramuscular and intraperitoneal injection of a spore suspension. The 13th day after intramuscular infection, a marked elevation of neutrophils was recorded in the peripheral blood, this involved a proliferation of band forms and segmented forms. As far as we know, this is the first report of infection due to E.pisciphila in Cardinal tetra.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10606 - Microbiology

Projekt

<a href="/cs/project/ED1.1.00%2F02.0109" target="_blank" >ED1.1.00/02.0109: Biotechnologické a biomedicínské centrum Akademie věd a Univerzity Karlovy</a><br>

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2017

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Journal of Fish Diseases

ISSN

0140-7775

e-ISSN

—

Svazek periodika

40

Číslo periodika v rámci svazku

8

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

101

Strana od-do

1015-1024

Kód UT WoS článku

000404934000003

EID výsledku v databázi Scopus

2-s2.0-85006969488