Lipidomic analysis of two closely related strains of the microalga Parietochloris (Trebouxiophyceae, Chlorophyta)

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388971%3A_____%2F17%3A00477524" target="_blank" >RIV/61388971:_____/17:00477524 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/67985939:_____/17:00477524 RIV/00216208:11310/17:10370071

Výsledek na webu

<a href="http://dx.doi.org/10.1016/j.algal.2017.06.005" target="_blank" >http://dx.doi.org/10.1016/j.algal.2017.06.005</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.algal.2017.06.005" target="_blank" >10.1016/j.algal.2017.06.005</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Lipidomic analysis of two closely related strains of the microalga Parietochloris (Trebouxiophyceae, Chlorophyta)

Popis výsledku v původním jazyce

The analysis of ribosomal internal transcribed spacer 2 (ITS2) secondary structure and morphology of two strains of the green microalga Parietochloris (CCALA 1082 and 1084) confirmed that they represent one biological species. However, their fatty acid profiles showed slight differences, which was fully confirmed by a series of lipidomic analyses using both shotgun lipidomics and hydrophilic interaction liquid chromatography-tandem mass spectrometry with electrospray ionization. 26 classes of lipids were identified, including more than 430 molecular species of lipids. The analyses showed that the greatest differences are in three types of phospholipids -phosphatidylethanolamine, phosphatidylcholine and phosphatidylinositol. These phospholipids have different spatial configurations (cylindrical, conical, and conical inverted), especially for those molecular species containing at least one arachidonic acid. Based on the lipidomic analyses we were able to distinguish the two strains. This may be advantageous in cases when routine DNA analysis shows that two strains are nearly identical.

Název v anglickém jazyce

Lipidomic analysis of two closely related strains of the microalga Parietochloris (Trebouxiophyceae, Chlorophyta)

Popis výsledku anglicky

The analysis of ribosomal internal transcribed spacer 2 (ITS2) secondary structure and morphology of two strains of the green microalga Parietochloris (CCALA 1082 and 1084) confirmed that they represent one biological species. However, their fatty acid profiles showed slight differences, which was fully confirmed by a series of lipidomic analyses using both shotgun lipidomics and hydrophilic interaction liquid chromatography-tandem mass spectrometry with electrospray ionization. 26 classes of lipids were identified, including more than 430 molecular species of lipids. The analyses showed that the greatest differences are in three types of phospholipids -phosphatidylethanolamine, phosphatidylcholine and phosphatidylinositol. These phospholipids have different spatial configurations (cylindrical, conical, and conical inverted), especially for those molecular species containing at least one arachidonic acid. Based on the lipidomic analyses we were able to distinguish the two strains. This may be advantageous in cases when routine DNA analysis shows that two strains are nearly identical.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10606 - Microbiology

Projekt

<a href="/cs/project/GA17-00027S" target="_blank" >GA17-00027S: Velmi dlouhé mastné kyseliny z mikroorganizmů</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2017

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Algal Research-Biomass Biofuels and Bioproducts

ISSN

2211-9264

e-ISSN

—

Svazek periodika

25

Číslo periodika v rámci svazku

JUL 2017

Stát vydavatele periodika

NL - Nizozemsko

Počet stran výsledku

10

Strana od-do

473-482

Kód UT WoS článku

000406305900049

EID výsledku v databázi Scopus

2-s2.0-85021058937