Identification and Characterization of Phospholipids with Very Long Chain Fatty Acids in Brewer's Yeast

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388971%3A_____%2F17%3A00482844" target="_blank" >RIV/61388971:_____/17:00482844 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/60461373:22330/17:43913249

Výsledek na webu

<a href="http://dx.doi.org/10.1007/s11745-017-4294-6" target="_blank" >http://dx.doi.org/10.1007/s11745-017-4294-6</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1007/s11745-017-4294-6" target="_blank" >10.1007/s11745-017-4294-6</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Identification and Characterization of Phospholipids with Very Long Chain Fatty Acids in Brewer's Yeast

Popis výsledku v původním jazyce

Yeast lipids and fatty acids (FA) were analyzed in Saccharomyces pastorianus from seven breweries and in the dietary yeast supplement Pangamin. GC-MS identified more than 30 FA, half of which were very-long chain fatty acids (VLCFA) with hydrocarbon chain lengths of ae<yen>22 C atoms. Positional isomers omega-9 and omega-7 were identified in FA with C18-C28 even-numbered alkyl chains. The most abundant omega-7 isomer was cis-vaccenic acid. The structure of monounsaturated FA was proved by dimethyl disulfide adducts (position of double bonds and cis geometric configuration) and by GC-MS of pyridyl carbinol esters. Ultra-high performance liquid chromatography-tandem mass spectrometry with negative electrospray ionization identified the phospholipids phosphatidylethanolamine, phosphatidylinositol and phosphatidylcholine, with more than 150 molecular species. Wild-type unmutated brewer's yeast strains conventionally used for the manufacture of food supplements were found to contain VLCFA.

Název v anglickém jazyce

Identification and Characterization of Phospholipids with Very Long Chain Fatty Acids in Brewer's Yeast

Popis výsledku anglicky

Yeast lipids and fatty acids (FA) were analyzed in Saccharomyces pastorianus from seven breweries and in the dietary yeast supplement Pangamin. GC-MS identified more than 30 FA, half of which were very-long chain fatty acids (VLCFA) with hydrocarbon chain lengths of ae<yen>22 C atoms. Positional isomers omega-9 and omega-7 were identified in FA with C18-C28 even-numbered alkyl chains. The most abundant omega-7 isomer was cis-vaccenic acid. The structure of monounsaturated FA was proved by dimethyl disulfide adducts (position of double bonds and cis geometric configuration) and by GC-MS of pyridyl carbinol esters. Ultra-high performance liquid chromatography-tandem mass spectrometry with negative electrospray ionization identified the phospholipids phosphatidylethanolamine, phosphatidylinositol and phosphatidylcholine, with more than 150 molecular species. Wild-type unmutated brewer's yeast strains conventionally used for the manufacture of food supplements were found to contain VLCFA.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10606 - Microbiology

Projekt

<a href="/cs/project/GA17-00027S" target="_blank" >GA17-00027S: Velmi dlouhé mastné kyseliny z mikroorganizmů</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2017

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Lipids

ISSN

0024-4201

e-ISSN

—

Svazek periodika

52

Číslo periodika v rámci svazku

12

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

11

Strana od-do

1007-1017

Kód UT WoS článku

000415878300005

EID výsledku v databázi Scopus

2-s2.0-85029459669