The synergistic effect of Selenium (selenite, -SeO32-) dose and irradiance intensity in Chlorella cultures

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388971%3A_____%2F17%3A00507324" target="_blank" >RIV/61388971:_____/17:00507324 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/60076658:12310/17:43895468

Výsledek na webu

<a href="https://amb-express.springeropen.com/articles/10.1186/s13568-017-0348-7" target="_blank" >https://amb-express.springeropen.com/articles/10.1186/s13568-017-0348-7</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1186/s13568-017-0348-7" target="_blank" >10.1186/s13568-017-0348-7</a>

Jazyk výsledku

angličtina

Název v původním jazyce

The synergistic effect of Selenium (selenite, -SeO32-) dose and irradiance intensity in Chlorella cultures

Popis výsledku v původním jazyce

Microalgae are able to metabolize inorganic selenium (Se) to organic forms (e.g. Se-proteins) nevertheless at certain Se concentration culture growth is inhibited. The aim of this work was to confirm the hypothesis that the limit of Se tolerance in Chlorella cultures is related to photosynthetic performance, i.e. depends on light intensity. We studied the relation between the dose and irradiance to find the range of Se tolerance in laboratory and outdoor cultures. At low irradiance (250 mu mol photons m(-2) s(-1)), the daily dose of Se below 8.5 mg per g of biomass (< 20 mu M) partially stimulated the photosynthetic activity (relative electron transport rate) and growth of Chlorella cultures (biomass density of similar to 1.5 g DW L-1) compared to the control (no Se added). It was accompanied by substantial Se incorporation to microalgae biomass (similar to 0.5 mg Se g(-1) DW). When the Se daily dose and level of irradiance were doubled (16 mg Se g(-1) DW 500 mu mol photons m(-2) s(-1)), the photosynthetic activity and growth were stimulated for several days and ample incorporation of Se to biomass (7.1 mg g(-1) DW) was observed. Yet, the same Se daily dose under increased irradiance (750 mu mol photons m(-2) s(-1)) caused the synergistic effect manifested by significant inhibition of photosynthesis, growth and lowered Se incorporation to biomass. In the present experiments Chl fluorescence techniques were used to monitor photosynthetic activity for determination of optimal Se doses in order to achieve efficient incorporation without substantial inhibition of microalgae growth when producing Se-enriched biomass.

Název v anglickém jazyce

The synergistic effect of Selenium (selenite, -SeO32-) dose and irradiance intensity in Chlorella cultures

Popis výsledku anglicky

Microalgae are able to metabolize inorganic selenium (Se) to organic forms (e.g. Se-proteins) nevertheless at certain Se concentration culture growth is inhibited. The aim of this work was to confirm the hypothesis that the limit of Se tolerance in Chlorella cultures is related to photosynthetic performance, i.e. depends on light intensity. We studied the relation between the dose and irradiance to find the range of Se tolerance in laboratory and outdoor cultures. At low irradiance (250 mu mol photons m(-2) s(-1)), the daily dose of Se below 8.5 mg per g of biomass (< 20 mu M) partially stimulated the photosynthetic activity (relative electron transport rate) and growth of Chlorella cultures (biomass density of similar to 1.5 g DW L-1) compared to the control (no Se added). It was accompanied by substantial Se incorporation to microalgae biomass (similar to 0.5 mg Se g(-1) DW). When the Se daily dose and level of irradiance were doubled (16 mg Se g(-1) DW 500 mu mol photons m(-2) s(-1)), the photosynthetic activity and growth were stimulated for several days and ample incorporation of Se to biomass (7.1 mg g(-1) DW) was observed. Yet, the same Se daily dose under increased irradiance (750 mu mol photons m(-2) s(-1)) caused the synergistic effect manifested by significant inhibition of photosynthesis, growth and lowered Se incorporation to biomass. In the present experiments Chl fluorescence techniques were used to monitor photosynthetic activity for determination of optimal Se doses in order to achieve efficient incorporation without substantial inhibition of microalgae growth when producing Se-enriched biomass.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10606 - Microbiology

Projekt

<a href="/cs/project/LO1416" target="_blank" >LO1416: Algatech plus</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2017

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

AMB Express

ISSN

2191-0855

e-ISSN

—

Svazek periodika

7

Číslo periodika v rámci svazku

MAR 7

Stát vydavatele periodika

GB - Spojené království Velké Británie a Severního Irska

Počet stran výsledku

14

Strana od-do

56

Kód UT WoS článku

000395980800001

EID výsledku v databázi Scopus

2-s2.0-85014780026