Application of spectrally resolved fluorescence induction to study light-induced nonphotochemical quenching in algae

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388971%3A_____%2F18%3A00489361" target="_blank" >RIV/61388971:_____/18:00489361 - isvavai.cz</a>

Výsledek na webu

<a href="http://dx.doi.org/10.1007/s11099-018-0780-1" target="_blank" >http://dx.doi.org/10.1007/s11099-018-0780-1</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1007/s11099-018-0780-1" target="_blank" >10.1007/s11099-018-0780-1</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Application of spectrally resolved fluorescence induction to study light-induced nonphotochemical quenching in algae

Popis výsledku v původním jazyce

The light-induced nonphotochemical quenching (NPQ) can safely dissipate excess of absorbed light to heat. Here we describe an application of spectrally resolved fluorescence induction (SRFI) method for studying spectral variability of NPQ. The approach allows detection of spectrally-resolved nonphotochemical quenching (NPQ(lambda)) representing NPQ dependency on fluorescence emission wavelength in the whole spectral range of fluorescence emission. The experimental approach is briefly described and NPQ(lambda) is studied for the cryptophyte alga Rhodomonas salina and for green alga Chlorella sp. We confirm presence of NPQ(lambda) only in membrane-bound antennae (chlorophyll a/c antennae) and not in phycobiliproteins in lumen in cryptophyte and show that NPQ(lambda) is inhibited in the whole spectral range by NPQ inhibitors in Chlorella sp. We discuss variability in the quenching in the particular spectral ranges and applicability of the NPQ(lambda) parameter to study quenching locus in vivo.

Název v anglickém jazyce

Application of spectrally resolved fluorescence induction to study light-induced nonphotochemical quenching in algae

Popis výsledku anglicky

The light-induced nonphotochemical quenching (NPQ) can safely dissipate excess of absorbed light to heat. Here we describe an application of spectrally resolved fluorescence induction (SRFI) method for studying spectral variability of NPQ. The approach allows detection of spectrally-resolved nonphotochemical quenching (NPQ(lambda)) representing NPQ dependency on fluorescence emission wavelength in the whole spectral range of fluorescence emission. The experimental approach is briefly described and NPQ(lambda) is studied for the cryptophyte alga Rhodomonas salina and for green alga Chlorella sp. We confirm presence of NPQ(lambda) only in membrane-bound antennae (chlorophyll a/c antennae) and not in phycobiliproteins in lumen in cryptophyte and show that NPQ(lambda) is inhibited in the whole spectral range by NPQ inhibitors in Chlorella sp. We discuss variability in the quenching in the particular spectral ranges and applicability of the NPQ(lambda) parameter to study quenching locus in vivo.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10606 - Microbiology

Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2018

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Photosynthetica

ISSN

0300-3604

e-ISSN

—

Svazek periodika

56

Číslo periodika v rámci svazku

1

Stát vydavatele periodika

CZ - Česká republika

Počet stran výsledku

7

Strana od-do

132-138

Kód UT WoS článku

000430309200011

EID výsledku v databázi Scopus

2-s2.0-85040378224