Antibiotic Resistance in Czech Urban Wastewater Treatment Plants: Microbial and Molecular Genetic Characterization

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388971%3A_____%2F18%3A00492597" target="_blank" >RIV/61388971:_____/18:00492597 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/00216208:11310/18:10382521

Výsledek na webu

<a href="http://dx.doi.org/10.1089/mdr.2017.0406" target="_blank" >http://dx.doi.org/10.1089/mdr.2017.0406</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1089/mdr.2017.0406" target="_blank" >10.1089/mdr.2017.0406</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Antibiotic Resistance in Czech Urban Wastewater Treatment Plants: Microbial and Molecular Genetic Characterization

Popis výsledku v původním jazyce

Quantitative changes in antibiotic resistance genes (ARGs) were investigated in six urban wastewater treatment plants (WWTPs) treating municipal and industrial wastewaters. In a selected WWTP, the fate of ARGs was studied in a 1-year time interval and in two phases of wastewater treatment process. Nine ARGs (tetW, tetO, tetA, tetB, tetM, bla(TEM), ermB, sul1, and intl1) were quantified in total and their relative abundance assessed by ARG copies/16SrRNA copies. From the tetracycline resistance genes, tetW was the only one detected in all sampled WWTPs. Its relative abundance in the nitrification tank of WWTP5 was found stable during the 1-year period, but was lowered by secondary sedimentation processes in the wastewater treatment down to 24% compared to the nitrification tank. Bacterial isolates showing high tetracycline resistance (minimal inhibition concentrations >100g/mL) were identified as members of Acinetobacter, Klebsiella, Citrobacter, Bacillus, and Enterobacter genera. Dynamic shifts in the relative abundance of ermB and sul1 were also demonstrated in wastewater samples from WWTP5.

Název v anglickém jazyce

Antibiotic Resistance in Czech Urban Wastewater Treatment Plants: Microbial and Molecular Genetic Characterization

Popis výsledku anglicky

Quantitative changes in antibiotic resistance genes (ARGs) were investigated in six urban wastewater treatment plants (WWTPs) treating municipal and industrial wastewaters. In a selected WWTP, the fate of ARGs was studied in a 1-year time interval and in two phases of wastewater treatment process. Nine ARGs (tetW, tetO, tetA, tetB, tetM, bla(TEM), ermB, sul1, and intl1) were quantified in total and their relative abundance assessed by ARG copies/16SrRNA copies. From the tetracycline resistance genes, tetW was the only one detected in all sampled WWTPs. Its relative abundance in the nitrification tank of WWTP5 was found stable during the 1-year period, but was lowered by secondary sedimentation processes in the wastewater treatment down to 24% compared to the nitrification tank. Bacterial isolates showing high tetracycline resistance (minimal inhibition concentrations >100g/mL) were identified as members of Acinetobacter, Klebsiella, Citrobacter, Bacillus, and Enterobacter genera. Dynamic shifts in the relative abundance of ermB and sul1 were also demonstrated in wastewater samples from WWTP5.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10606 - Microbiology

Projekt

—

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2018

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Microbial Drug Resistance-Mechanisms Epidemiology and Disease

ISSN

1076-6294

e-ISSN

—

Svazek periodika

24

Číslo periodika v rámci svazku

6

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

9

Strana od-do

830-838

Kód UT WoS článku

000434481100001

EID výsledku v databázi Scopus

2-s2.0-85049878546