Magnesium Stable Isotope Fractionation on a Cellular Level Explored by Cyanobacteria and Black Fungi with Implications for Higher Plants

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388971%3A_____%2F18%3A00498448" target="_blank" >RIV/61388971:_____/18:00498448 - isvavai.cz</a>

Výsledek na webu

<a href="http://dx.doi.org/10.1021/acs.est.8b02238" target="_blank" >http://dx.doi.org/10.1021/acs.est.8b02238</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1021/acs.est.8b02238" target="_blank" >10.1021/acs.est.8b02238</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Magnesium Stable Isotope Fractionation on a Cellular Level Explored by Cyanobacteria and Black Fungi with Implications for Higher Plants

Popis výsledku v původním jazyce

In a controlled growth experiment we found that the cyanobacterium Nostoc punctiforme has a bulk cell Mg-26/Mg-24 ratio (expressed as delta Mg-26) that is -0.27 parts per thousand lower than the growth solution at a pH of ca. 5.9. This contrasts with a recently published delta Mg-26 value that was 0.65 parts per thousand higher than growth solution for the black fungus Knufia petricola at similar laboratory conditions, interpreted to reflect loss of Mg-24 during cell growth. By a mass balance model constrained by delta Mg-26 in chlorophyll extract we inferred the delta Mg-26 value of the main Mg compartments in a cyanobacteria cell: free cytosolic Mg (-2.64 parts per thousand), chlorophyll (1.85 parts per thousand), and the nonchlorophyll-bonded Mg compartments like ATP and ribosomes (-0.64%o). The lower delta Mg-26 found in Nostoc punctiforme would thus result from the absence of significant Mg efflux during cell growth in combination with either (a) discrimination against Mg-26 during uptake by desolvation of Mg or transport across protein channels or (b) discrimination against Mg-24 in the membrane transporter during efflux. The model predicts the preferential incorporation of Mg-26 in cells and plant organs low in Mg and the absence of isotope fractionation in those high in Mg, corroborated by a compilation of Mg isotope ratios from fungi, bacteria, and higher plants.

Název v anglickém jazyce

Magnesium Stable Isotope Fractionation on a Cellular Level Explored by Cyanobacteria and Black Fungi with Implications for Higher Plants

Popis výsledku anglicky

In a controlled growth experiment we found that the cyanobacterium Nostoc punctiforme has a bulk cell Mg-26/Mg-24 ratio (expressed as delta Mg-26) that is -0.27 parts per thousand lower than the growth solution at a pH of ca. 5.9. This contrasts with a recently published delta Mg-26 value that was 0.65 parts per thousand higher than growth solution for the black fungus Knufia petricola at similar laboratory conditions, interpreted to reflect loss of Mg-24 during cell growth. By a mass balance model constrained by delta Mg-26 in chlorophyll extract we inferred the delta Mg-26 value of the main Mg compartments in a cyanobacteria cell: free cytosolic Mg (-2.64 parts per thousand), chlorophyll (1.85 parts per thousand), and the nonchlorophyll-bonded Mg compartments like ATP and ribosomes (-0.64%o). The lower delta Mg-26 found in Nostoc punctiforme would thus result from the absence of significant Mg efflux during cell growth in combination with either (a) discrimination against Mg-26 during uptake by desolvation of Mg or transport across protein channels or (b) discrimination against Mg-24 in the membrane transporter during efflux. The model predicts the preferential incorporation of Mg-26 in cells and plant organs low in Mg and the absence of isotope fractionation in those high in Mg, corroborated by a compilation of Mg isotope ratios from fungi, bacteria, and higher plants.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10618 - Ecology

Projekt

<a href="/cs/project/LO1416" target="_blank" >LO1416: Algatech plus</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2018

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Environmental Science & Technology Letters

ISSN

2328-8930

e-ISSN

—

Svazek periodika

52

Číslo periodika v rámci svazku

21

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

9

Strana od-do

12216-12224

Kód UT WoS článku

000449722200026

EID výsledku v databázi Scopus

2-s2.0-85056131304