Nanodiamonds as Effective Vectors for Micro RNA-Based Drug Delivery

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388971%3A_____%2F18%3A00499815" target="_blank" >RIV/61388971:_____/18:00499815 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Nanodiamonds as Effective Vectors for Micro RNA-Based Drug Delivery

Popis výsledku v původním jazyce

Micro RNAs are short non-coding RNA molecules, which are important regulators of cell functions. Deregulation of certain micro RNA level is contributing factor to tumor formation and progression. Possible gene therapy by modulation of the micro RNA level is currently considered. Recently it has been proved that nano diamonds coated with PEI serve as efficient transfection and monitoring system. The aim of our work was to deliver micro RNA inhibitor and micro RNA mimic sequences into specialized mammalianncells using fluorescent nano diamond particles and to test the nano complex uptake and cell compatibility during primary in vivo application. Short RNA molecules were bound to the PEI coated nano diamonds, transported into the colon cancer cell line in vitro and observed in a confocal microscope. The miRNA mimic coated nano diamonds were used for complex uptake and toxicity study in peritoneal macrophages and splenocytes after ex vivo stimulation or in vivo administration. Due to the possibility of continuousnmonitoring of carrier internalization, its transfection efficacy, and cell compatibility, the fluorescent nano diamonds represent a suitable RNA carrier with a potential use in gene therapy.

Název v anglickém jazyce

Nanodiamonds as Effective Vectors for Micro RNA-Based Drug Delivery

Popis výsledku anglicky

Micro RNAs are short non-coding RNA molecules, which are important regulators of cell functions. Deregulation of certain micro RNA level is contributing factor to tumor formation and progression. Possible gene therapy by modulation of the micro RNA level is currently considered. Recently it has been proved that nano diamonds coated with PEI serve as efficient transfection and monitoring system. The aim of our work was to deliver micro RNA inhibitor and micro RNA mimic sequences into specialized mammalianncells using fluorescent nano diamond particles and to test the nano complex uptake and cell compatibility during primary in vivo application. Short RNA molecules were bound to the PEI coated nano diamonds, transported into the colon cancer cell line in vitro and observed in a confocal microscope. The miRNA mimic coated nano diamonds were used for complex uptake and toxicity study in peritoneal macrophages and splenocytes after ex vivo stimulation or in vivo administration. Due to the possibility of continuousnmonitoring of carrier internalization, its transfection efficacy, and cell compatibility, the fluorescent nano diamonds represent a suitable RNA carrier with a potential use in gene therapy.

Druh

J_ost - Ostatní články v recenzovaných periodicích

CEP obor

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OECD FORD obor

10606 - Microbiology

Projekt

<a href="/cs/project/NV15-33094A" target="_blank" >NV15-33094A: Nanovlákenné nosiče léčiv s řízeným uvolněním pro hojení ran založené na enkapsulaci funkcionalizovaných nanodiamantových částic</a><br>

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2018

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Drug Designing & Intellectual Properties International Journal

ISSN

2637-4706

e-ISSN

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Svazek periodika

1

Číslo periodika v rámci svazku

4

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

6

Strana od-do

112-117

Kód UT WoS článku

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EID výsledku v databázi Scopus

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