Kocuria Bacterial Isolates from Radioactive Springs of Jachymov spa (Joachimsthal) as Sources of Polyunsaturated Fatty Acids

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388971%3A_____%2F19%3A00503944" target="_blank" >RIV/61388971:_____/19:00503944 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/60461373:22330/19:43917820

Výsledek na webu

<a href="https://aocs.onlinelibrary.wiley.com/doi/abs/10.1002/lipd.12136" target="_blank" >https://aocs.onlinelibrary.wiley.com/doi/abs/10.1002/lipd.12136</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1002/lipd.12136" target="_blank" >10.1002/lipd.12136</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Kocuria Bacterial Isolates from Radioactive Springs of Jachymov spa (Joachimsthal) as Sources of Polyunsaturated Fatty Acids

Popis výsledku v původním jazyce

Four bacterial isolates, which produced polyunsaturated fatty acids (PUFA), were isolated from water samples of radioactive springs collected from Jachymov spa. Jachymov (Sankt Joachimsthal) is a city in northwestern Bohemia, where Marie and Pierre Curie isolated radium in 1898 from the mineral uraninite. To date, four springs (Agricola, Behounek, C1, and Curie) have been used for spa purposes, that is for the treatment of nervous and rheumatic disorders by constantly produced radioactive gas radon (Rn-222) dissolved in the water. The radioactivity reaches 24 kBq/L. Using 16S rRNA gene sequence analysis, all four isolates were identified as members of the genus Kocuria, with two isolates designated 208 and 401 affiliated with Kocuria kristinae, while isolates 101 and 301 most likely with K. rhizophila. The content of fatty acids in polar lipids was determined by gas chromatography-mass spectrometry (GC-MS) and two PUFA, that is arachidonic and eicosapentaenoic, were identified. The position of double bonds was confirmed by GC-MS of 3-pyridylcarbinol (formerly picolinyl) esters. We assume that all four isolates of Kocuria produce PUFA to increase the stability of cell membranes, which may be impaired by the reaction of the reactive oxygen species. These can arise, for example, because of radiation during Rn-222 decay.

Název v anglickém jazyce

Kocuria Bacterial Isolates from Radioactive Springs of Jachymov spa (Joachimsthal) as Sources of Polyunsaturated Fatty Acids

Popis výsledku anglicky

Four bacterial isolates, which produced polyunsaturated fatty acids (PUFA), were isolated from water samples of radioactive springs collected from Jachymov spa. Jachymov (Sankt Joachimsthal) is a city in northwestern Bohemia, where Marie and Pierre Curie isolated radium in 1898 from the mineral uraninite. To date, four springs (Agricola, Behounek, C1, and Curie) have been used for spa purposes, that is for the treatment of nervous and rheumatic disorders by constantly produced radioactive gas radon (Rn-222) dissolved in the water. The radioactivity reaches 24 kBq/L. Using 16S rRNA gene sequence analysis, all four isolates were identified as members of the genus Kocuria, with two isolates designated 208 and 401 affiliated with Kocuria kristinae, while isolates 101 and 301 most likely with K. rhizophila. The content of fatty acids in polar lipids was determined by gas chromatography-mass spectrometry (GC-MS) and two PUFA, that is arachidonic and eicosapentaenoic, were identified. The position of double bonds was confirmed by GC-MS of 3-pyridylcarbinol (formerly picolinyl) esters. We assume that all four isolates of Kocuria produce PUFA to increase the stability of cell membranes, which may be impaired by the reaction of the reactive oxygen species. These can arise, for example, because of radiation during Rn-222 decay.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10606 - Microbiology

Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2019

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Lipids

ISSN

0024-4201

e-ISSN

—

Svazek periodika

54

Číslo periodika v rámci svazku

2-3

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

11

Strana od-do

177-187

Kód UT WoS článku

000462095000006

EID výsledku v databázi Scopus

2-s2.0-85062794497