Preclinical Alternative Model for Analysis of Porous Scaffold Biocompatibility Applicable in Bone Tissue Engineering

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388971%3A_____%2F19%3A00504321" target="_blank" >RIV/61388971:_____/19:00504321 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/67985823:_____/19:00504321 RIV/00216208:11110/19:10398420

Výsledek na webu

<a href="https://doi.org/10.14573/altex.1807241" target="_blank" >https://doi.org/10.14573/altex.1807241</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.14573/altex.1807241" target="_blank" >10.14573/altex.1807241</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Preclinical Alternative Model for Analysis of Porous Scaffold Biocompatibility Applicable in Bone Tissue Engineering

Popis výsledku v původním jazyce

Porous scaffolds represent a potential approach to repair critical-size bone defects. Vascularization is essential for bone formation and healing. This study establishes a method to monitor angiogenesis within porous biopolymer scaffolds made on the basis of polyhydroxybutyrate and chitosan. We used the chick and quail chorioallantoic membrane (CAM) assay as an alternative in vivo model to study the formation of new blood vessels inside the scaffold structure. The chemical properties of the biopolymer scaffold matrix surface were characterized as well as the tissue reaction of the CAM. Placing a piece of polymer scaffold on the CAM resulted in a vascular reaction documented visually and by ultrasound biomicroscopy. Histological analysis showed a myofibroblast reaction (smooth muscle actin-positive cells) without excessive collagen deposition. Cell invasion into the implant was observed and the presence of a vascular network was confirmed by identifying hemangioblasts and endothelial cells of quail origin using the QH1 marker. The CAM assay is a rapid and easy way to test biocompatibility and vasculogenic potential of new candidate scaffolds for bone tissue bioengineering while respecting the 3Rs.

Název v anglickém jazyce

Preclinical Alternative Model for Analysis of Porous Scaffold Biocompatibility Applicable in Bone Tissue Engineering

Popis výsledku anglicky

Porous scaffolds represent a potential approach to repair critical-size bone defects. Vascularization is essential for bone formation and healing. This study establishes a method to monitor angiogenesis within porous biopolymer scaffolds made on the basis of polyhydroxybutyrate and chitosan. We used the chick and quail chorioallantoic membrane (CAM) assay as an alternative in vivo model to study the formation of new blood vessels inside the scaffold structure. The chemical properties of the biopolymer scaffold matrix surface were characterized as well as the tissue reaction of the CAM. Placing a piece of polymer scaffold on the CAM resulted in a vascular reaction documented visually and by ultrasound biomicroscopy. Histological analysis showed a myofibroblast reaction (smooth muscle actin-positive cells) without excessive collagen deposition. Cell invasion into the implant was observed and the presence of a vascular network was confirmed by identifying hemangioblasts and endothelial cells of quail origin using the QH1 marker. The CAM assay is a rapid and easy way to test biocompatibility and vasculogenic potential of new candidate scaffolds for bone tissue bioengineering while respecting the 3Rs.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10606 - Microbiology

Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

—

Rok uplatnění

2019

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

ALTEX-Alternatives to Animal Experimentation

ISSN

1868-596X

e-ISSN

—

Svazek periodika

36

Číslo periodika v rámci svazku

1

Stát vydavatele periodika

DE - Spolková republika Německo

Počet stran výsledku

10

Strana od-do

121-130

Kód UT WoS článku

000455105700010

EID výsledku v databázi Scopus

2-s2.0-85059914227