Tracking of the activity of individual bacteria in temperate forest soils shows guild-specific responses to seasonality

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388971%3A_____%2F19%3A00510382" target="_blank" >RIV/61388971:_____/19:00510382 - isvavai.cz</a>

Výsledek na webu

<a href="https://www.sciencedirect.com/science/article/pii/S0038071719301440?via%3Dihub" target="_blank" >https://www.sciencedirect.com/science/article/pii/S0038071719301440?via%3Dihub</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.soilbio.2019.05.010" target="_blank" >10.1016/j.soilbio.2019.05.010</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Tracking of the activity of individual bacteria in temperate forest soils shows guild-specific responses to seasonality

Popis výsledku v původním jazyce

Bacteria belong to the key players in the carbon cycling in forest soils and thus affect the global C balance. Our understanding of bacterial contribution to ecosystem processes relies on the analysis of whole communities. Here we combined strain isolation, genome sequencing and metatranscriptomics to analyse the activity of individual bacterial species in the topsoil of a temperate coniferous forest. Our results show that transcription profiles in litter and soil differ, indicating that bacterial activity is shaped by their habitat. Importantly, transcript pools also significant differ between summer when primary producers are active and winter. We show that Acidobacteria and Bacteroidetes with high metabolic capacity for polysaccharide decomposition in their genomes actively transcribe corresponding genes in situ. The rate of the transcription of ribosomal proteins, a proxy of growth, seasonally differs between these 'decomposer' taxa and other 'opportunistic' bacteria. While the former grow at similar rates in summer and winter, the latter grow much faster in summer when labile C, delivered by plant roots, is available. This paper demonstrates differences in activity of bacterial guilds as well as the importance of environmental drivers for the activity of individual bacteria.

Název v anglickém jazyce

Tracking of the activity of individual bacteria in temperate forest soils shows guild-specific responses to seasonality

Popis výsledku anglicky

Bacteria belong to the key players in the carbon cycling in forest soils and thus affect the global C balance. Our understanding of bacterial contribution to ecosystem processes relies on the analysis of whole communities. Here we combined strain isolation, genome sequencing and metatranscriptomics to analyse the activity of individual bacterial species in the topsoil of a temperate coniferous forest. Our results show that transcription profiles in litter and soil differ, indicating that bacterial activity is shaped by their habitat. Importantly, transcript pools also significant differ between summer when primary producers are active and winter. We show that Acidobacteria and Bacteroidetes with high metabolic capacity for polysaccharide decomposition in their genomes actively transcribe corresponding genes in situ. The rate of the transcription of ribosomal proteins, a proxy of growth, seasonally differs between these 'decomposer' taxa and other 'opportunistic' bacteria. While the former grow at similar rates in summer and winter, the latter grow much faster in summer when labile C, delivered by plant roots, is available. This paper demonstrates differences in activity of bacterial guilds as well as the importance of environmental drivers for the activity of individual bacteria.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10606 - Microbiology

Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2019

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Soil Biology and Biochemistry

ISSN

0038-0717

e-ISSN

—

Svazek periodika

135

Číslo periodika v rámci svazku

AUG

Stát vydavatele periodika

GB - Spojené království Velké Británie a Severního Irska

Počet stran výsledku

8

Strana od-do

275-282

Kód UT WoS článku

000477689700032

EID výsledku v databázi Scopus

2-s2.0-85065883250