Observations on two microbial life strategies in soil: Planktonic and biofilm-forming microorganisms are separable

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388971%3A_____%2F19%3A00519359" target="_blank" >RIV/61388971:_____/19:00519359 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/44555601:13440/19:43894773 RIV/44555601:13510/19:43894773

Výsledek na webu

<a href="https://www.sciencedirect.com/science/article/pii/S0038071719301993" target="_blank" >https://www.sciencedirect.com/science/article/pii/S0038071719301993</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.soilbio.2019.107535" target="_blank" >10.1016/j.soilbio.2019.107535</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Observations on two microbial life strategies in soil: Planktonic and biofilm-forming microorganisms are separable

Popis výsledku v původním jazyce

Biofilms, the communities of sessile microbial forms, are hotspots of biological activity that coexist in soil together with free-living (planktonic) biota. Sessile and planktonic microbial communities may constitute functionally different groups of organisms with different roles in interactions with organic matter and plants. Nevertheless, soil microbiomes are regularly analyzed without distinguishing biofilm inhabitants and planktonic organisms. Consequently, basic information regarding taxa constituting both communities is severely lacking, which limits the understanding of the basic structure of soil microbiome and consequently also of the microbiome functioning.nIn this study, we tested the hypothesis that soil biofilm and planktonic microbial communities are different. Glass fiber filters were exposed to three different field soils for 12 weeks and biofilms arose on their surfaces. The biofilms were further separated from the planktonic forms by washing the latter out of the filters and both communities were analyzed using next generation sequencing. The results revealed significant differences between biofilm and planktonic communities of bacteria and eukaryotic organisms. Our data indicate common production of motile microbial cells in the soil and specialization of some taxa (Legionella spp.) to planktonic life mode. We also noted an association between the abundance of some bacterial taxa and eukaryotic grazers suggesting a trophic interaction. Tillage, as a cause of soil disturbance, did not result in a significant increase in the abundance of most abundant biofilm associated microbial taxa. This is the first analysis of separated sessile (biofilm) and motile (planktonic) communities of soil microorganisms.

Název v anglickém jazyce

Observations on two microbial life strategies in soil: Planktonic and biofilm-forming microorganisms are separable

Popis výsledku anglicky

Biofilms, the communities of sessile microbial forms, are hotspots of biological activity that coexist in soil together with free-living (planktonic) biota. Sessile and planktonic microbial communities may constitute functionally different groups of organisms with different roles in interactions with organic matter and plants. Nevertheless, soil microbiomes are regularly analyzed without distinguishing biofilm inhabitants and planktonic organisms. Consequently, basic information regarding taxa constituting both communities is severely lacking, which limits the understanding of the basic structure of soil microbiome and consequently also of the microbiome functioning.nIn this study, we tested the hypothesis that soil biofilm and planktonic microbial communities are different. Glass fiber filters were exposed to three different field soils for 12 weeks and biofilms arose on their surfaces. The biofilms were further separated from the planktonic forms by washing the latter out of the filters and both communities were analyzed using next generation sequencing. The results revealed significant differences between biofilm and planktonic communities of bacteria and eukaryotic organisms. Our data indicate common production of motile microbial cells in the soil and specialization of some taxa (Legionella spp.) to planktonic life mode. We also noted an association between the abundance of some bacterial taxa and eukaryotic grazers suggesting a trophic interaction. Tillage, as a cause of soil disturbance, did not result in a significant increase in the abundance of most abundant biofilm associated microbial taxa. This is the first analysis of separated sessile (biofilm) and motile (planktonic) communities of soil microorganisms.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10606 - Microbiology

Projekt

<a href="/cs/project/GA17-09946S" target="_blank" >GA17-09946S: Půdní biofilmy tvořené spolupracujícími mikroorganismy: jejich vznik a dynamika v heterogenním prostředí</a><br>

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2019

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Soil Biology and Biochemistry

ISSN

0038-0717

e-ISSN

—

Svazek periodika

136

Číslo periodika v rámci svazku

SEP 2019

Stát vydavatele periodika

GB - Spojené království Velké Británie a Severního Irska

Počet stran výsledku

11

Strana od-do

—

Kód UT WoS článku

000483908600020

EID výsledku v databázi Scopus

2-s2.0-85068909442