Characterization of Immune Cell Subset Expansion in Response to Therapeutic Treatment in Mice

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388971%3A_____%2F20%3A00559265" target="_blank" >RIV/61388971:_____/20:00559265 - isvavai.cz</a>

Výsledek na webu

<a href="http://dx.doi.org/10.1007/978-1-0716-0266-9_9" target="_blank" >http://dx.doi.org/10.1007/978-1-0716-0266-9_9</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1007/978-1-0716-0266-9_9" target="_blank" >10.1007/978-1-0716-0266-9_9</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Characterization of Immune Cell Subset Expansion in Response to Therapeutic Treatment in Mice

Popis výsledku v původním jazyce

Flow cytometry has revolutionized the field of molecular immunology, enabling the monitoring and characterization of immune events at the single-cell level. Here, we describe a flow cytometry-based workflow to quantify the activation of specific immune cell subsets in mice in response to a molecular intervention. Compared to laborious long-term disease models, this technique allows for relatively rapid evaluation of candidate therapeutics designed to elicit a targeted immune response. This approach has the range to address both disease applications in which an immunostimulatory effect would be desired (e.g., cancer, infectious disease) or those in which an immunosuppressive effect would be desired (e.g., autoimmune disorders, transplantation medicine). Overall, our technique presents a powerful and accessible strategy for preliminary in vivo assessment of potential immunotherapeutics.

Název v anglickém jazyce

Characterization of Immune Cell Subset Expansion in Response to Therapeutic Treatment in Mice

Popis výsledku anglicky

Flow cytometry has revolutionized the field of molecular immunology, enabling the monitoring and characterization of immune events at the single-cell level. Here, we describe a flow cytometry-based workflow to quantify the activation of specific immune cell subsets in mice in response to a molecular intervention. Compared to laborious long-term disease models, this technique allows for relatively rapid evaluation of candidate therapeutics designed to elicit a targeted immune response. This approach has the range to address both disease applications in which an immunostimulatory effect would be desired (e.g., cancer, infectious disease) or those in which an immunosuppressive effect would be desired (e.g., autoimmune disorders, transplantation medicine). Overall, our technique presents a powerful and accessible strategy for preliminary in vivo assessment of potential immunotherapeutics.

Druh

C - Kapitola v odborné knize

CEP obor

—

OECD FORD obor

30102 - Immunology

Projekt

—

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2020

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název knihy nebo sborníku

T-Cell Receptor Signaling Methods and Protocols

ISBN

978-1-0716-0268-3

Počet stran výsledku

14

Strana od-do

101-114

Počet stran knihy

295

Název nakladatele

HUMANA PRESS INC

Místo vydání

Totowa

Kód UT WoS kapitoly

000674446600010