Studying Protein-DNA Interactions by Hydrogen/Deuterium Exchange Mass Spectrometry

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388971%3A_____%2F21%3A00537940" target="_blank" >RIV/61388971:_____/21:00537940 - isvavai.cz</a>

Výsledek na webu

<a href="https://link.springer.com/protocol/10.1007/978-1-0716-1126-5_11" target="_blank" >https://link.springer.com/protocol/10.1007/978-1-0716-1126-5_11</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1007/978-1-0716-1126-5_11" target="_blank" >10.1007/978-1-0716-1126-5_11</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Studying Protein-DNA Interactions by Hydrogen/Deuterium Exchange Mass Spectrometry

Popis výsledku v původním jazyce

Protein hydrogen/deuterium exchange (HDX) coupled to mass spectrometry (MS) can be used to study interactions of proteins with various ligands, to describe the effects of mutations, or to reveal structural responses of proteins to different experimental conditions. It is often described as a method with virtually no limitations in terms of protein size or sample composition. While this is generally true, there are, however, ligands or buffer components that can significantly complicate the analysis. One such compound, that can make HDX-MS troublesome, is DNA. In this chapter, we will focus on the analysis of protein–DNA interactions, describe the detailed protocol, and point out ways to overcome the complications arising from the presence of DNA.

Název v anglickém jazyce

Studying Protein-DNA Interactions by Hydrogen/Deuterium Exchange Mass Spectrometry

Popis výsledku anglicky

Protein hydrogen/deuterium exchange (HDX) coupled to mass spectrometry (MS) can be used to study interactions of proteins with various ligands, to describe the effects of mutations, or to reveal structural responses of proteins to different experimental conditions. It is often described as a method with virtually no limitations in terms of protein size or sample composition. While this is generally true, there are, however, ligands or buffer components that can significantly complicate the analysis. One such compound, that can make HDX-MS troublesome, is DNA. In this chapter, we will focus on the analysis of protein–DNA interactions, describe the detailed protocol, and point out ways to overcome the complications arising from the presence of DNA.

Druh

C - Kapitola v odborné knize

CEP obor

—

OECD FORD obor

10608 - Biochemistry and molecular biology

Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2021

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název knihy nebo sborníku

Multiprotein Complexes

ISBN

978-1-0716-1125-8

Počet stran výsledku

27

Strana od-do

193-219

Počet stran knihy

340

Název nakladatele

Springer

Místo vydání

New York

Kód UT WoS kapitoly

000685659700012