Physiological and spectroscopical changes of the thermophilic cyanobacterium Synechococcus elongatus under iron stress and recovery culture

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388971%3A_____%2F21%3A00542474" target="_blank" >RIV/61388971:_____/21:00542474 - isvavai.cz</a>

Výsledek na webu

<a href="https://link.springer.com/article/10.1007/s11738-021-03242-0" target="_blank" >https://link.springer.com/article/10.1007/s11738-021-03242-0</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1007/s11738-021-03242-0" target="_blank" >10.1007/s11738-021-03242-0</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Physiological and spectroscopical changes of the thermophilic cyanobacterium Synechococcus elongatus under iron stress and recovery culture

Popis výsledku v původním jazyce

The iron in cyanobacterial cells contributes to energy conversion via electron transport chains, so its availability directly influences all metabolic pathways. Besides exploring cell adaptations and changes occurring with iron insufficient stress, the present work examines profoundly for the first time the metabolic changes accompanied with iron recovery for the thermophilic cyanobacterium Synechococcus elongatus (Nag., var. thermalis Geitl. Strain Kovrov 1972/8). S. elongatus cells were cultivated on two iron deprivation levels, iron-limited (45 nM Fe) and iron-deficient (4.5 nM Fe). Growth, carotenoids/Chlorophyll-a (Car/Chl-a) ratio, transmitting electron images, pigments fractionation analysis, and cell activity were checked. The obtained results demonstrated an astounding decrease in Chl-a content, an increment in beta-carotene content, leading to rising the Car/Chl-a ratio, a reduction of phycobilins content, a reduction in cell diameters, a decrease of energy transfer, a depletion of the electron transport chain, and a reduction of photosynthesis and respiration processes under inadequate iron condition. Likewise, the photochemical activity of photosystem II (PSII), determined by Fv/Fm ratio and thermoluminescence estimations, demonstrated that PSII was disabled under iron pressure. With iron recovery by 200 nM Fe, cells regained full metabolic activity within 24 h. Despite the fact that photosynthesis and respiration activity exhibited nearly a similar behavior, S. elongatus under iron-deficiency began to recuperate the activity of the photosynthetic apparatus quicker than the respiratory machinery by regaining their pigment content and activity.

Název v anglickém jazyce

Physiological and spectroscopical changes of the thermophilic cyanobacterium Synechococcus elongatus under iron stress and recovery culture

Popis výsledku anglicky

The iron in cyanobacterial cells contributes to energy conversion via electron transport chains, so its availability directly influences all metabolic pathways. Besides exploring cell adaptations and changes occurring with iron insufficient stress, the present work examines profoundly for the first time the metabolic changes accompanied with iron recovery for the thermophilic cyanobacterium Synechococcus elongatus (Nag., var. thermalis Geitl. Strain Kovrov 1972/8). S. elongatus cells were cultivated on two iron deprivation levels, iron-limited (45 nM Fe) and iron-deficient (4.5 nM Fe). Growth, carotenoids/Chlorophyll-a (Car/Chl-a) ratio, transmitting electron images, pigments fractionation analysis, and cell activity were checked. The obtained results demonstrated an astounding decrease in Chl-a content, an increment in beta-carotene content, leading to rising the Car/Chl-a ratio, a reduction of phycobilins content, a reduction in cell diameters, a decrease of energy transfer, a depletion of the electron transport chain, and a reduction of photosynthesis and respiration processes under inadequate iron condition. Likewise, the photochemical activity of photosystem II (PSII), determined by Fv/Fm ratio and thermoluminescence estimations, demonstrated that PSII was disabled under iron pressure. With iron recovery by 200 nM Fe, cells regained full metabolic activity within 24 h. Despite the fact that photosynthesis and respiration activity exhibited nearly a similar behavior, S. elongatus under iron-deficiency began to recuperate the activity of the photosynthetic apparatus quicker than the respiratory machinery by regaining their pigment content and activity.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10606 - Microbiology

Projekt

<a href="/cs/project/GA18-07822S" target="_blank" >GA18-07822S: Strategie adaptace eukaryotického fytoplanktonu na nedostatek železa</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2021

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Acta Physiologiae Plantarum

ISSN

0137-5881

e-ISSN

1861-1664

Svazek periodika

43

Číslo periodika v rámci svazku

5

Stát vydavatele periodika

DE - Spolková republika Německo

Počet stran výsledku

11

Strana od-do

72

Kód UT WoS článku

000641849900001

EID výsledku v databázi Scopus

2-s2.0-85104513918