Local angiotensin-converting enzyme 2 gene expression in kidney allografts is not affected by renin-angiotensin-aldosterone inhibitors

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388971%3A_____%2F21%3A00542902" target="_blank" >RIV/61388971:_____/21:00542902 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/00023001:_____/21:00081047

Výsledek na webu

<a href="https://www.karger.com/Article/Abstract/513710" target="_blank" >https://www.karger.com/Article/Abstract/513710</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1159/000513710" target="_blank" >10.1159/000513710</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Local angiotensin-converting enzyme 2 gene expression in kidney allografts is not affected by renin-angiotensin-aldosterone inhibitors

Popis výsledku v původním jazyce

Background: Preclinical studies suggested that pharmacological inhibition of the renin-angiotensin-aldosterone system (RAAS) by ACE inhibitors (ACEis) or angiotensin II receptor blockers (ARBs) may increase local angiotensin-converting enzyme 2 (ACE2) expression. Methods: In this study, we evaluated the effect of ACEi or ARB treatment on expression of ACE2, ACE, and AGTR1 in 3-month protocol kidney allograft biopsies of stable patients using RT-qPCR (n = 48). Protein ACE2 expression was assessed using immunohistochemistry from paraffin sections. Results: The therapy with RAAS blockers was not associated with increased ACE2, ACE, or ATGR1 expression in kidney allografts and also ACE2 protein immunohistochemistry did not reveal differences among groups. Conclusions: ACEis or ARBs in kidney transplant recipients do not affect local ACE2 expression. This observation supports long-term RAAS treatment in kidney transplant recipients, despite acute complications such as COVID-19 where ACE2 serves as the entry protein for infection.

Název v anglickém jazyce

Local angiotensin-converting enzyme 2 gene expression in kidney allografts is not affected by renin-angiotensin-aldosterone inhibitors

Popis výsledku anglicky

Background: Preclinical studies suggested that pharmacological inhibition of the renin-angiotensin-aldosterone system (RAAS) by ACE inhibitors (ACEis) or angiotensin II receptor blockers (ARBs) may increase local angiotensin-converting enzyme 2 (ACE2) expression. Methods: In this study, we evaluated the effect of ACEi or ARB treatment on expression of ACE2, ACE, and AGTR1 in 3-month protocol kidney allograft biopsies of stable patients using RT-qPCR (n = 48). Protein ACE2 expression was assessed using immunohistochemistry from paraffin sections. Results: The therapy with RAAS blockers was not associated with increased ACE2, ACE, or ATGR1 expression in kidney allografts and also ACE2 protein immunohistochemistry did not reveal differences among groups. Conclusions: ACEis or ARBs in kidney transplant recipients do not affect local ACE2 expression. This observation supports long-term RAAS treatment in kidney transplant recipients, despite acute complications such as COVID-19 where ACE2 serves as the entry protein for infection.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10606 - Microbiology

Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2021

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Kidney & Blood Pressure Research

ISSN

1420-4096

e-ISSN

1423-0143

Svazek periodika

46

Číslo periodika v rámci svazku

2

Stát vydavatele periodika

CH - Švýcarská konfederace

Počet stran výsledku

5

Strana od-do

245-249

Kód UT WoS článku

000632540000001

EID výsledku v databázi Scopus

2-s2.0-85103322183