Diclofenac Alters the Cell Cycle Progression of the Green Alga Chlamydomonas reinhardtii

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388971%3A_____%2F21%3A00546629" target="_blank" >RIV/61388971:_____/21:00546629 - isvavai.cz</a>

Výsledek na webu

<a href="https://www.mdpi.com/2073-4409/10/8/1936" target="_blank" >https://www.mdpi.com/2073-4409/10/8/1936</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.3390/cells10081936" target="_blank" >10.3390/cells10081936</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Diclofenac Alters the Cell Cycle Progression of the Green Alga Chlamydomonas reinhardtii

Popis výsledku v původním jazyce

The aim of the study was to verify the hypothesis that a potential cause of the phytotoxicity of diclofenac (DCF, a non-steroidal anti-inflammatory drug) is an effect of cell cycle progression. This research was conducted using synchronous cultures of a model organism, green alga Chlamydomonas reinhardtii. The project examined DCF effects on selected parameters that characterize cell cycle progression, such as cell size, attainment of commitment points, DNA replication, number of nuclei formed during cells division and morphology of cells in consecutive stages of the cell cycle, together with the physiological and biochemical parameters of algae cells at different stages. We demonstrated that individual cell growth remained unaffected, whereas cell division was delayed in the DCF-treated groups grown in continuous light conditions, and the number of daughter cells from a single cell decreased. Thus, the cell cycle progression is a target affected by DCF, which has a similar anti-proliferative effect on mammalian cells.

Název v anglickém jazyce

Diclofenac Alters the Cell Cycle Progression of the Green Alga Chlamydomonas reinhardtii

Popis výsledku anglicky

The aim of the study was to verify the hypothesis that a potential cause of the phytotoxicity of diclofenac (DCF, a non-steroidal anti-inflammatory drug) is an effect of cell cycle progression. This research was conducted using synchronous cultures of a model organism, green alga Chlamydomonas reinhardtii. The project examined DCF effects on selected parameters that characterize cell cycle progression, such as cell size, attainment of commitment points, DNA replication, number of nuclei formed during cells division and morphology of cells in consecutive stages of the cell cycle, together with the physiological and biochemical parameters of algae cells at different stages. We demonstrated that individual cell growth remained unaffected, whereas cell division was delayed in the DCF-treated groups grown in continuous light conditions, and the number of daughter cells from a single cell decreased. Thus, the cell cycle progression is a target affected by DCF, which has a similar anti-proliferative effect on mammalian cells.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10606 - Microbiology

Projekt

<a href="/cs/project/GA19-12607S" target="_blank" >GA19-12607S: Růst a buněčný cyklus – regulace vstupu do buněčného cyklu a výstupu z něj</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2021

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Cells

ISSN

2073-4409

e-ISSN

2073-4409

Svazek periodika

10

Číslo periodika v rámci svazku

8

Stát vydavatele periodika

CH - Švýcarská konfederace

Počet stran výsledku

15

Strana od-do

1936

Kód UT WoS článku

000688980300001

EID výsledku v databázi Scopus

2-s2.0-85115041946