Nucleotide composition bias of rDNA sequences as a source of phylogenetic artifacts in Basidiomycota-a case of a new lineage of a uredinicolous Ramularia-like anamorph with affinities to Ustilaginomycotina

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388971%3A_____%2F21%3A00549670" target="_blank" >RIV/61388971:_____/21:00549670 - isvavai.cz</a>

Výsledek na webu

<a href="https://link.springer.com/article/10.1007%2Fs11557-021-01749-x" target="_blank" >https://link.springer.com/article/10.1007%2Fs11557-021-01749-x</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1007/s11557-021-01749-x" target="_blank" >10.1007/s11557-021-01749-x</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Nucleotide composition bias of rDNA sequences as a source of phylogenetic artifacts in Basidiomycota-a case of a new lineage of a uredinicolous Ramularia-like anamorph with affinities to Ustilaginomycotina

Popis výsledku v původním jazyce

A Ramularia-like hyphomycete was discovered on uredinia of Phakopsora ampelopsidis on leaves of wild Ampelopsis brevipedunculata and cultivated Parthenocissus tricuspidata in several cities in Taiwan. The micromorphology of this fungicolous fungus is similar to that of species of the genus Ramularia which are mostly plant parasites but some species grow on rust fungi. Analyses of SSU and LSU rDNA and RPB2 gene sequence data and of ultrastructural features observed by scanning and transmission electron microscopy revealed that the fungus represents a novel lineage within the Ustilaginomycotina. The name Quasiramularia phakopsoricola is proposed for this new species in a new genus, a new family, and a new order. Sequence data obtained for ITS1, 5.8S, and ITS2 rDNA, however, did not match to any known fungal lineage. Bioinformatics analyses showed that these sequence data are extremely GC poor, but most probably do not represent a pseudogene. Extreme deviations of GC content can be observed in several lineages of Basidiomycota. Such variations affect the results of phylogenetic analyses and are an important source of artifacts.

Název v anglickém jazyce

Nucleotide composition bias of rDNA sequences as a source of phylogenetic artifacts in Basidiomycota-a case of a new lineage of a uredinicolous Ramularia-like anamorph with affinities to Ustilaginomycotina

Popis výsledku anglicky

A Ramularia-like hyphomycete was discovered on uredinia of Phakopsora ampelopsidis on leaves of wild Ampelopsis brevipedunculata and cultivated Parthenocissus tricuspidata in several cities in Taiwan. The micromorphology of this fungicolous fungus is similar to that of species of the genus Ramularia which are mostly plant parasites but some species grow on rust fungi. Analyses of SSU and LSU rDNA and RPB2 gene sequence data and of ultrastructural features observed by scanning and transmission electron microscopy revealed that the fungus represents a novel lineage within the Ustilaginomycotina. The name Quasiramularia phakopsoricola is proposed for this new species in a new genus, a new family, and a new order. Sequence data obtained for ITS1, 5.8S, and ITS2 rDNA, however, did not match to any known fungal lineage. Bioinformatics analyses showed that these sequence data are extremely GC poor, but most probably do not represent a pseudogene. Extreme deviations of GC content can be observed in several lineages of Basidiomycota. Such variations affect the results of phylogenetic analyses and are an important source of artifacts.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10606 - Microbiology

Projekt

—

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2021

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Mycological Progress

ISSN

1617-416X

e-ISSN

1861-8952

Svazek periodika

20

Číslo periodika v rámci svazku

12

Stát vydavatele periodika

DE - Spolková republika Německo

Počet stran výsledku

19

Strana od-do

1553-1571

Kód UT WoS článku

000724834800003

EID výsledku v databázi Scopus

2-s2.0-85120733626