Trichloromethane dechlorination by a novel Dehalobacter sp. strain 8M reveals a third contrasting C and Cl isotope fractionation pattern within this genus

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388971%3A_____%2F22%3A00553502" target="_blank" >RIV/61388971:_____/22:00553502 - isvavai.cz</a>

Výsledek na webu

<a href="https://www.sciencedirect.com/science/article/pii/S0048969721077378?via%3Dihub" target="_blank" >https://www.sciencedirect.com/science/article/pii/S0048969721077378?via%3Dihub</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.scitotenv.2021.152659" target="_blank" >10.1016/j.scitotenv.2021.152659</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Trichloromethane dechlorination by a novel Dehalobacter sp. strain 8M reveals a third contrasting C and Cl isotope fractionation pattern within this genus

Popis výsledku v původním jazyce

Trichloromethane (TCM) is a pollutant frequently detected in contaminated aquifers, and only four bacterial strains are known to respire it. Here, we obtained a novel Dehalobacter strain capable of transforming TCM to dichloromethane, which was denominated Dehalobacter sp. strain 8M. Besides TCM, strain 8M also completely transformed 1,1,2-trichloroethane to vinyl chloride and 1,2-dichloroethane. Quantitative PCR analysis for the 16S rRNA genes confirmed growth of Dehalobacter with TCM and 1,1,2-trichloroethane as electron acceptors. Carbon and chlorine isotope fractionation during TCM transformation was studied in cultured cells and in enzymatic assays with cell suspensions and crude protein extracts. TCM transformation in the three studied systems resulted in small but significant carbon (epsilon(C) =2.7 +/- 0.1 parts per thousand for respiring cells,3.1 +/- 0.1 parts per thousand for cell suspensions, and 4.1 +/- 0.5 parts per thousand for crude protein extracts) and chlorine (epsilon(Cl) =0.9 +/- 0.1 parts per thousand,1.1 +/- 0.1 parts per thousand, and 1.2 +/- 0.2 parts per thousand, respectively) isotope fractionation. A characteristic and consistent dual CCl isotope fractionation pattern was observed for the three systems (combined Lambda(C/Cl) = 2.8 +/- 0.3). This Lambda(C/Cl) differed significantly from previously reported values for anaerobic dechlorination of TCM by the corrinoid cofactor vitamin B12 and other Dehalobacter strains. These findings widen our knowledge on the existence of different enzyme binding mechanisms underlying TCM-dechlorination within the genus Dehalobacter and demonstrates that dual isotope analysis could be a feasible tool to differentiate TCM degraders at field studies.

Název v anglickém jazyce

Trichloromethane dechlorination by a novel Dehalobacter sp. strain 8M reveals a third contrasting C and Cl isotope fractionation pattern within this genus

Popis výsledku anglicky

Trichloromethane (TCM) is a pollutant frequently detected in contaminated aquifers, and only four bacterial strains are known to respire it. Here, we obtained a novel Dehalobacter strain capable of transforming TCM to dichloromethane, which was denominated Dehalobacter sp. strain 8M. Besides TCM, strain 8M also completely transformed 1,1,2-trichloroethane to vinyl chloride and 1,2-dichloroethane. Quantitative PCR analysis for the 16S rRNA genes confirmed growth of Dehalobacter with TCM and 1,1,2-trichloroethane as electron acceptors. Carbon and chlorine isotope fractionation during TCM transformation was studied in cultured cells and in enzymatic assays with cell suspensions and crude protein extracts. TCM transformation in the three studied systems resulted in small but significant carbon (epsilon(C) =2.7 +/- 0.1 parts per thousand for respiring cells,3.1 +/- 0.1 parts per thousand for cell suspensions, and 4.1 +/- 0.5 parts per thousand for crude protein extracts) and chlorine (epsilon(Cl) =0.9 +/- 0.1 parts per thousand,1.1 +/- 0.1 parts per thousand, and 1.2 +/- 0.2 parts per thousand, respectively) isotope fractionation. A characteristic and consistent dual CCl isotope fractionation pattern was observed for the three systems (combined Lambda(C/Cl) = 2.8 +/- 0.3). This Lambda(C/Cl) differed significantly from previously reported values for anaerobic dechlorination of TCM by the corrinoid cofactor vitamin B12 and other Dehalobacter strains. These findings widen our knowledge on the existence of different enzyme binding mechanisms underlying TCM-dechlorination within the genus Dehalobacter and demonstrates that dual isotope analysis could be a feasible tool to differentiate TCM degraders at field studies.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10606 - Microbiology

Projekt

—

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2022

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Science of the Total Environment

ISSN

0048-9697

e-ISSN

1879-1026

Svazek periodika

813

Číslo periodika v rámci svazku

MAR 20 2022

Stát vydavatele periodika

NL - Nizozemsko

Počet stran výsledku

9

Strana od-do

152659

Kód UT WoS článku

000748713900012

EID výsledku v databázi Scopus

2-s2.0-85121909144