Fatal affairs - conjugational transfer of a dinoflagellate- killing plasmid between marine Rhodobacterales

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388971%3A_____%2F22%3A00557609" target="_blank" >RIV/61388971:_____/22:00557609 - isvavai.cz</a>

Výsledek na webu

<a href="https://www.microbiologyresearch.org/content/journal/mgen/10.1099/mgen.0.000787" target="_blank" >https://www.microbiologyresearch.org/content/journal/mgen/10.1099/mgen.0.000787</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1099/mgen.0.000787" target="_blank" >10.1099/mgen.0.000787</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Fatal affairs - conjugational transfer of a dinoflagellate- killing plasmid between marine Rhodobacterales

Popis výsledku v původním jazyce

The roseobacter group of marine bacteria is characterized by a mosaic distribution of ecologically important phenotypes. These are often encoded on mobile extrachromosomal replicons. So far, conjugation had only been experimentally proven between the two model organisms Phaeobacter inhibens and Dinoroseobacter shibae. Here, we show that two large natural RepABCtype plasmids from D. shibae can be transferred into representatives of all known major Rhodobacterales lineages. Complete genome sequencing of the newly established Phaeobacter inhibens transconjugants confirmed their genomic integrity. The conjugated plasmids were stably maintained as single copy number replicons in the genuine as well as the new host. Co- cultivation of Phaeobacter inhibens and the transconjugants with the dinoflagellate Prorocentrum minimum demonstrated that Phaeobacter inhibens is a probiotic strain that improves the yield and stability of the dinoflagellate culture. The transconjugant carrying the 191 kb plasmid, but not the 126 kb sister plasmid, killed the dinoflagellate in co- culture.

Název v anglickém jazyce

Fatal affairs - conjugational transfer of a dinoflagellate- killing plasmid between marine Rhodobacterales

Popis výsledku anglicky

The roseobacter group of marine bacteria is characterized by a mosaic distribution of ecologically important phenotypes. These are often encoded on mobile extrachromosomal replicons. So far, conjugation had only been experimentally proven between the two model organisms Phaeobacter inhibens and Dinoroseobacter shibae. Here, we show that two large natural RepABCtype plasmids from D. shibae can be transferred into representatives of all known major Rhodobacterales lineages. Complete genome sequencing of the newly established Phaeobacter inhibens transconjugants confirmed their genomic integrity. The conjugated plasmids were stably maintained as single copy number replicons in the genuine as well as the new host. Co- cultivation of Phaeobacter inhibens and the transconjugants with the dinoflagellate Prorocentrum minimum demonstrated that Phaeobacter inhibens is a probiotic strain that improves the yield and stability of the dinoflagellate culture. The transconjugant carrying the 191 kb plasmid, but not the 126 kb sister plasmid, killed the dinoflagellate in co- culture.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10606 - Microbiology

Projekt

—

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2022

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Microbial Genomics

ISSN

2057-5858

e-ISSN

2057-5858

Svazek periodika

8

Číslo periodika v rámci svazku

3

Stát vydavatele periodika

GB - Spojené království Velké Británie a Severního Irska

Počet stran výsledku

12

Strana od-do

000787

Kód UT WoS článku

000788985200003

EID výsledku v databázi Scopus

2-s2.0-85125871952