Microbial utilization of simple and complex carbon compounds in a temperate forest soil
Identifikátory výsledku
Kód výsledku v IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388971%3A_____%2F22%3A00560514" target="_blank" >RIV/61388971:_____/22:00560514 - isvavai.cz</a>
Nalezeny alternativní kódy
RIV/00216208:11310/22:10454569
Výsledek na webu
<a href="https://www.sciencedirect.com/science/article/pii/S0038071722002437?via%3Dihub" target="_blank" >https://www.sciencedirect.com/science/article/pii/S0038071722002437?via%3Dihub</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/j.soilbio.2022.108786" target="_blank" >10.1016/j.soilbio.2022.108786</a>
Alternativní jazyky
Jazyk výsledku
angličtina
Název v původním jazyce
Microbial utilization of simple and complex carbon compounds in a temperate forest soil
Popis výsledku v původním jazyce
Forest soil processes carried out by microorganisms are critical for the global carbon (C) cycle and climate. Characterizing the utilization of differently recalcitrant C sources is an important step towards understanding the ecosystem-level function of microorganisms in temperate forest soils. Here, using stable-isotope probing (SIP), we tracked C incorporation into bacterial and fungal biomass by quantifying 13C incorporation into phospholipid fatty acids (PLFA-SIP), its respiration (i.e., content in the produced CO2) and C accumulation by individual microbial taxa (DNA-SIP), following the addition of 13C-labelled substrates of different recalcitrance (citric acid, glucose, chitin, cellulose, hemicellulose, and plant biomass) in microcosms. The highest 13C respiration was observed after the addition of the low-molecular-mass substrates citric acid and glucose, while the highest 13C incorporation into microbial biomass was observed during growth on chitin. Communities of fungi and bacteria that incorporated 13C of various origins into their biomass differed from the original soil communities, as well as between treatments. The most distinct microbial community was observed in microcosms containing 13C-chitin, indicating its utilization by both fungi and bacteria. Bacterial taxa were more often versatile, incorporating C of various origins, while there was a higher share of fungi that were specialists. Together, our results show that lowmolecular-mass compounds that belong to typical root exudates are more readily respired, while the C from biopolymers studied was relatively more incorporated into microbial biomass. Various C sources are targeted by distinct microbial communities, although their composition partly overlaps due to the existence of generalist bacteria and fungi that are capable of utilizing various C sources.
Název v anglickém jazyce
Microbial utilization of simple and complex carbon compounds in a temperate forest soil
Popis výsledku anglicky
Forest soil processes carried out by microorganisms are critical for the global carbon (C) cycle and climate. Characterizing the utilization of differently recalcitrant C sources is an important step towards understanding the ecosystem-level function of microorganisms in temperate forest soils. Here, using stable-isotope probing (SIP), we tracked C incorporation into bacterial and fungal biomass by quantifying 13C incorporation into phospholipid fatty acids (PLFA-SIP), its respiration (i.e., content in the produced CO2) and C accumulation by individual microbial taxa (DNA-SIP), following the addition of 13C-labelled substrates of different recalcitrance (citric acid, glucose, chitin, cellulose, hemicellulose, and plant biomass) in microcosms. The highest 13C respiration was observed after the addition of the low-molecular-mass substrates citric acid and glucose, while the highest 13C incorporation into microbial biomass was observed during growth on chitin. Communities of fungi and bacteria that incorporated 13C of various origins into their biomass differed from the original soil communities, as well as between treatments. The most distinct microbial community was observed in microcosms containing 13C-chitin, indicating its utilization by both fungi and bacteria. Bacterial taxa were more often versatile, incorporating C of various origins, while there was a higher share of fungi that were specialists. Together, our results show that lowmolecular-mass compounds that belong to typical root exudates are more readily respired, while the C from biopolymers studied was relatively more incorporated into microbial biomass. Various C sources are targeted by distinct microbial communities, although their composition partly overlaps due to the existence of generalist bacteria and fungi that are capable of utilizing various C sources.
Klasifikace
Druh
J<sub>imp</sub> - Článek v periodiku v databázi Web of Science
CEP obor
—
OECD FORD obor
10606 - Microbiology
Návaznosti výsledku
Projekt
<a href="/cs/project/GA22-30769S" target="_blank" >GA22-30769S: Cyklus uhlíku v půdách a globální změny: vliv depozice dusíku</a><br>
Návaznosti
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Ostatní
Rok uplatnění
2022
Kód důvěrnosti údajů
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Údaje specifické pro druh výsledku
Název periodika
Soil Biology and Biochemistry
ISSN
0038-0717
e-ISSN
—
Svazek periodika
173
Číslo periodika v rámci svazku
October 2022
Stát vydavatele periodika
GB - Spojené království Velké Británie a Severního Irska
Počet stran výsledku
10
Strana od-do
108786
Kód UT WoS článku
000843493300001
EID výsledku v databázi Scopus
2-s2.0-85135684009