Distribution of phosphorus cycling genes across land uses and microbial taxonomic groups based on metagenome and genome mining

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388971%3A_____%2F22%3A00562728" target="_blank" >RIV/61388971:_____/22:00562728 - isvavai.cz</a>

Výsledek na webu

<a href="https://www.sciencedirect.com/science/article/pii/S0038071722002838?via%3Dihub" target="_blank" >https://www.sciencedirect.com/science/article/pii/S0038071722002838?via%3Dihub</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.soilbio.2022.108826" target="_blank" >10.1016/j.soilbio.2022.108826</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Distribution of phosphorus cycling genes across land uses and microbial taxonomic groups based on metagenome and genome mining

Popis výsledku v původním jazyce

Phosphorus (P) is an essential and limiting nutrient in soil and is tightly linked to soil fertility and productivity. Microorganisms have developed different mechanisms to respond to P scarcity and increase its availability in soil, which are susceptible to change under contrasting land uses. Here, we calculated and compared meta-genomic redundancy, as a measurement of ecosystem potential capacity, of 23 key functional genes related to organic P mineralization, inorganic P solubilization and P-starvation response regulation in forest, grassland and cropland soils through mining in public sequence repository. The redundancy of those genes in all currently published genomes (genome redundancy) from archaea, bacteria and fungi was also studied. Microbes in croplands and grasslands showed a higher potential (i.e., redundancy) to mineralize organic P through the action of alkaline phosphatases (phoA, phoD and phoX genes) and to solubilize inorganic P (gcd and pqqC) by producing gluconic acid than those in forests. Instead, the capacity of microbes to mineralize phosphonates through the action of C-P lyases (phnG, phnH, ..., phnM) was found to be higher in forests. The impact of land use on the metagenomic redundancy of genes encoding phytases (appA and 3-phytase) was dependent on the type of phytase. Intermetagenome redundancy (potentiality per metagenome unit) reached maximum values for phos-phatase production, P solubilization and regulation of P starvation, denoting the crucial role that these functions have in P cycling. Proteobacteria, within Bacteria, and Euryarchaeota, within Archaea, showed the greatest genomic potential to respond to P scarcity. However, the role of fungi seems to be more restricted. The present study provides an overview on how the microbial mechanisms that regulate P availability in soil potentially change with land use and taxonomy of microbes.

Název v anglickém jazyce

Distribution of phosphorus cycling genes across land uses and microbial taxonomic groups based on metagenome and genome mining

Popis výsledku anglicky

Phosphorus (P) is an essential and limiting nutrient in soil and is tightly linked to soil fertility and productivity. Microorganisms have developed different mechanisms to respond to P scarcity and increase its availability in soil, which are susceptible to change under contrasting land uses. Here, we calculated and compared meta-genomic redundancy, as a measurement of ecosystem potential capacity, of 23 key functional genes related to organic P mineralization, inorganic P solubilization and P-starvation response regulation in forest, grassland and cropland soils through mining in public sequence repository. The redundancy of those genes in all currently published genomes (genome redundancy) from archaea, bacteria and fungi was also studied. Microbes in croplands and grasslands showed a higher potential (i.e., redundancy) to mineralize organic P through the action of alkaline phosphatases (phoA, phoD and phoX genes) and to solubilize inorganic P (gcd and pqqC) by producing gluconic acid than those in forests. Instead, the capacity of microbes to mineralize phosphonates through the action of C-P lyases (phnG, phnH, ..., phnM) was found to be higher in forests. The impact of land use on the metagenomic redundancy of genes encoding phytases (appA and 3-phytase) was dependent on the type of phytase. Intermetagenome redundancy (potentiality per metagenome unit) reached maximum values for phos-phatase production, P solubilization and regulation of P starvation, denoting the crucial role that these functions have in P cycling. Proteobacteria, within Bacteria, and Euryarchaeota, within Archaea, showed the greatest genomic potential to respond to P scarcity. However, the role of fungi seems to be more restricted. The present study provides an overview on how the microbial mechanisms that regulate P availability in soil potentially change with land use and taxonomy of microbes.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10606 - Microbiology

Projekt

—

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2022

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Soil Biology and Biochemistry

ISSN

0038-0717

e-ISSN

—

Svazek periodika

174

Číslo periodika v rámci svazku

November 2022

Stát vydavatele periodika

GB - Spojené království Velké Británie a Severního Irska

Počet stran výsledku

8

Strana od-do

108826

Kód UT WoS článku

000861293800001

EID výsledku v databázi Scopus

2-s2.0-85137734404