Photosynthesis Monitoring in Microalgae Cultures Grown on Municipal Wastewater as a Nutrient Source in Large-Scale Outdoor Bioreactors

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388971%3A_____%2F22%3A00563739" target="_blank" >RIV/61388971:_____/22:00563739 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/60076658:12310/22:43904947

Výsledek na webu

<a href="https://www.mdpi.com/2079-7737/11/10/1380" target="_blank" >https://www.mdpi.com/2079-7737/11/10/1380</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.3390/biology11101380" target="_blank" >10.3390/biology11101380</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Photosynthesis Monitoring in Microalgae Cultures Grown on Municipal Wastewater as a Nutrient Source in Large-Scale Outdoor Bioreactors

Popis výsledku v původním jazyce

Simple Summary The remediation of waste nutrients, mainly nitrogen, phosphorous, and carbon, results in low-cost biomass production. In this work, raceway ponds with an area of 1 and 0.5 ha were built in Merida, Spain next to a municipal wastewater (WW) treatment plant. These DEMO bioreactors are operated continuously all year round. At the start of the trials, the bioreactors were filled with urban WW as a sole source of nutrients and left to be populated by the sewage-born phytoplankton. The fitness and growth of the phytoplankton population (dominated by the green microalga Micractinium) were monitored by photosynthesis measuring techniques. The cultures showed suitable photosynthetic activity. Productivity values ranged between 15 and 25 g DW m(-2) day(-1) and processed biomass revealed biostimulating activity. In these trials, photosynthesis measuring techniques, i.e., oxygen evolution and chlorophyll (Chl) fluorescence techniques, were validated to monitor large-scale bioreactors using municipal WW remediation for biomass production, which can be used for agricultural purposes as biofertilizer/biostimulant. Microalgae cultures were used for a WW treatment to remediate nutrients while producing biomass and recycling water. In these trials, raceway ponds (RWPs, 1 and 0.5 ha) were located next to a municipal (WW) treatment plant in Merida, Spain. The ponds were used for continuous, all-year-round microalgae production using WW as a source of nutrients. Neither CO2 nor air was supplied to cultures. The objective was to validate photosynthesis monitoring techniques in large-scale bioreactors. Various in-situ/ex-situ methods based on chlorophyll fluorescence and oxygen evolution measurements were used to follow culture performance. Photosynthesis variables gathered with these techniques were compared to the physiological behavior and growth of cultures. Good photosynthetic activity was indicated by the build-up of dissolved oxygen concentration up to 380% saturation, high photochemical yield (Fv/Fm = 0.62-0.71), and relative electron transport rate rETR between 200 and 450 mu mol e(-) m(-2) s(-1) at midday, which resulted in biomass productivity of about 15-25 g DW m(-2) day(-1). The variables represent reliable markers reflecting the physiological status of microalgae cultures. Using waste nutrients, the biomass production cost can be significantly decreased for abundant biomass production in large-scale bioreactors, which can be exploited for agricultural purposes.

Název v anglickém jazyce

Photosynthesis Monitoring in Microalgae Cultures Grown on Municipal Wastewater as a Nutrient Source in Large-Scale Outdoor Bioreactors

Popis výsledku anglicky

Simple Summary The remediation of waste nutrients, mainly nitrogen, phosphorous, and carbon, results in low-cost biomass production. In this work, raceway ponds with an area of 1 and 0.5 ha were built in Merida, Spain next to a municipal wastewater (WW) treatment plant. These DEMO bioreactors are operated continuously all year round. At the start of the trials, the bioreactors were filled with urban WW as a sole source of nutrients and left to be populated by the sewage-born phytoplankton. The fitness and growth of the phytoplankton population (dominated by the green microalga Micractinium) were monitored by photosynthesis measuring techniques. The cultures showed suitable photosynthetic activity. Productivity values ranged between 15 and 25 g DW m(-2) day(-1) and processed biomass revealed biostimulating activity. In these trials, photosynthesis measuring techniques, i.e., oxygen evolution and chlorophyll (Chl) fluorescence techniques, were validated to monitor large-scale bioreactors using municipal WW remediation for biomass production, which can be used for agricultural purposes as biofertilizer/biostimulant. Microalgae cultures were used for a WW treatment to remediate nutrients while producing biomass and recycling water. In these trials, raceway ponds (RWPs, 1 and 0.5 ha) were located next to a municipal (WW) treatment plant in Merida, Spain. The ponds were used for continuous, all-year-round microalgae production using WW as a source of nutrients. Neither CO2 nor air was supplied to cultures. The objective was to validate photosynthesis monitoring techniques in large-scale bioreactors. Various in-situ/ex-situ methods based on chlorophyll fluorescence and oxygen evolution measurements were used to follow culture performance. Photosynthesis variables gathered with these techniques were compared to the physiological behavior and growth of cultures. Good photosynthetic activity was indicated by the build-up of dissolved oxygen concentration up to 380% saturation, high photochemical yield (Fv/Fm = 0.62-0.71), and relative electron transport rate rETR between 200 and 450 mu mol e(-) m(-2) s(-1) at midday, which resulted in biomass productivity of about 15-25 g DW m(-2) day(-1). The variables represent reliable markers reflecting the physiological status of microalgae cultures. Using waste nutrients, the biomass production cost can be significantly decreased for abundant biomass production in large-scale bioreactors, which can be exploited for agricultural purposes.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10606 - Microbiology

Projekt

—

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2022

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Biology

ISSN

2079-7737

e-ISSN

2079-7737

Svazek periodika

11

Číslo periodika v rámci svazku

10

Stát vydavatele periodika

CH - Švýcarská konfederace

Počet stran výsledku

18

Strana od-do

1380

Kód UT WoS článku

000872351500001

EID výsledku v databázi Scopus

2-s2.0-85140386728