Fatty acid 16:1 omega 5 as a proxy for arbuscular mycorrhizal fungal biomass: current challenges and ways forward

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388971%3A_____%2F22%3A00564055" target="_blank" >RIV/61388971:_____/22:00564055 - isvavai.cz</a>

Výsledek na webu

<a href="https://link.springer.com/article/10.1007/s00374-022-01670-9" target="_blank" >https://link.springer.com/article/10.1007/s00374-022-01670-9</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1007/s00374-022-01670-9" target="_blank" >10.1007/s00374-022-01670-9</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Fatty acid 16:1 omega 5 as a proxy for arbuscular mycorrhizal fungal biomass: current challenges and ways forward

Popis výsledku v původním jazyce

Fatty acid biomarkers have emerged as a useful tool to quantify biomass of various microbial groups. Here we focus on the frequent use of the fatty acid 16:1 omega 5 as a biomarker for arbuscular mycorrhizal (AM) fungi in soils. We highlight some issues with current applications of this method and use several examples from the literature to show that the phospholipid fatty acid (PLFA) 16:1 omega 5 can occur in high concentrations in soils where actively growing AM fungi are absent. Unless the study includes a control where the contribution of other microbes can be estimated, we advocate for the use of the neutral lipid fatty acid (NLFA) 16:1 omega 5. This biomarker has higher specificity, is more responsive to shifts in AM fungal biomass, and quantification can be conducted along with PLFA analysis without doubling analytical efforts. We conclude by contrasting various methods used to measure AM fungal biomass in soil and highlight future research needs to optimize fatty acid analyses.

Název v anglickém jazyce

Fatty acid 16:1 omega 5 as a proxy for arbuscular mycorrhizal fungal biomass: current challenges and ways forward

Popis výsledku anglicky

Fatty acid biomarkers have emerged as a useful tool to quantify biomass of various microbial groups. Here we focus on the frequent use of the fatty acid 16:1 omega 5 as a biomarker for arbuscular mycorrhizal (AM) fungi in soils. We highlight some issues with current applications of this method and use several examples from the literature to show that the phospholipid fatty acid (PLFA) 16:1 omega 5 can occur in high concentrations in soils where actively growing AM fungi are absent. Unless the study includes a control where the contribution of other microbes can be estimated, we advocate for the use of the neutral lipid fatty acid (NLFA) 16:1 omega 5. This biomarker has higher specificity, is more responsive to shifts in AM fungal biomass, and quantification can be conducted along with PLFA analysis without doubling analytical efforts. We conclude by contrasting various methods used to measure AM fungal biomass in soil and highlight future research needs to optimize fatty acid analyses.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10606 - Microbiology

Projekt

—

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2022

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Biology and Fertility of Soils

ISSN

0178-2762

e-ISSN

1432-0789

Svazek periodika

58

Číslo periodika v rámci svazku

8

Stát vydavatele periodika

NL - Nizozemsko

Počet stran výsledku

8

Strana od-do

835-842

Kód UT WoS článku

000865195700001

EID výsledku v databázi Scopus

2-s2.0-85139224187