Fatty acid 16:1 omega 5 as a proxy for arbuscular mycorrhizal fungal biomass: current challenges and ways forward
Identifikátory výsledku
Kód výsledku v IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388971%3A_____%2F22%3A00564055" target="_blank" >RIV/61388971:_____/22:00564055 - isvavai.cz</a>
Výsledek na webu
<a href="https://link.springer.com/article/10.1007/s00374-022-01670-9" target="_blank" >https://link.springer.com/article/10.1007/s00374-022-01670-9</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1007/s00374-022-01670-9" target="_blank" >10.1007/s00374-022-01670-9</a>
Alternativní jazyky
Jazyk výsledku
angličtina
Název v původním jazyce
Fatty acid 16:1 omega 5 as a proxy for arbuscular mycorrhizal fungal biomass: current challenges and ways forward
Popis výsledku v původním jazyce
Fatty acid biomarkers have emerged as a useful tool to quantify biomass of various microbial groups. Here we focus on the frequent use of the fatty acid 16:1 omega 5 as a biomarker for arbuscular mycorrhizal (AM) fungi in soils. We highlight some issues with current applications of this method and use several examples from the literature to show that the phospholipid fatty acid (PLFA) 16:1 omega 5 can occur in high concentrations in soils where actively growing AM fungi are absent. Unless the study includes a control where the contribution of other microbes can be estimated, we advocate for the use of the neutral lipid fatty acid (NLFA) 16:1 omega 5. This biomarker has higher specificity, is more responsive to shifts in AM fungal biomass, and quantification can be conducted along with PLFA analysis without doubling analytical efforts. We conclude by contrasting various methods used to measure AM fungal biomass in soil and highlight future research needs to optimize fatty acid analyses.
Název v anglickém jazyce
Fatty acid 16:1 omega 5 as a proxy for arbuscular mycorrhizal fungal biomass: current challenges and ways forward
Popis výsledku anglicky
Fatty acid biomarkers have emerged as a useful tool to quantify biomass of various microbial groups. Here we focus on the frequent use of the fatty acid 16:1 omega 5 as a biomarker for arbuscular mycorrhizal (AM) fungi in soils. We highlight some issues with current applications of this method and use several examples from the literature to show that the phospholipid fatty acid (PLFA) 16:1 omega 5 can occur in high concentrations in soils where actively growing AM fungi are absent. Unless the study includes a control where the contribution of other microbes can be estimated, we advocate for the use of the neutral lipid fatty acid (NLFA) 16:1 omega 5. This biomarker has higher specificity, is more responsive to shifts in AM fungal biomass, and quantification can be conducted along with PLFA analysis without doubling analytical efforts. We conclude by contrasting various methods used to measure AM fungal biomass in soil and highlight future research needs to optimize fatty acid analyses.
Klasifikace
Druh
J<sub>imp</sub> - Článek v periodiku v databázi Web of Science
CEP obor
—
OECD FORD obor
10606 - Microbiology
Návaznosti výsledku
Projekt
—
Návaznosti
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Ostatní
Rok uplatnění
2022
Kód důvěrnosti údajů
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Údaje specifické pro druh výsledku
Název periodika
Biology and Fertility of Soils
ISSN
0178-2762
e-ISSN
1432-0789
Svazek periodika
58
Číslo periodika v rámci svazku
8
Stát vydavatele periodika
NL - Nizozemsko
Počet stran výsledku
8
Strana od-do
835-842
Kód UT WoS článku
000865195700001
EID výsledku v databázi Scopus
2-s2.0-85139224187