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Specific utilization of biopolymers of plant and fungal origin reveals the existence of substrate-specific guilds for bacteria in temperate forest soils

Identifikátory výsledku

  • Kód výsledku v IS VaVaI

    <a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388971%3A_____%2F22%3A00565241" target="_blank" >RIV/61388971:_____/22:00565241 - isvavai.cz</a>

  • Výsledek na webu

    <a href="https://www.sciencedirect.com/science/article/pii/S0038071722001535?via%3Dihub" target="_blank" >https://www.sciencedirect.com/science/article/pii/S0038071722001535?via%3Dihub</a>

  • DOI - Digital Object Identifier

    <a href="http://dx.doi.org/10.1016/j.soilbio.2022.108696" target="_blank" >10.1016/j.soilbio.2022.108696</a>

Alternativní jazyky

  • Jazyk výsledku

    angličtina

  • Název v původním jazyce

    Specific utilization of biopolymers of plant and fungal origin reveals the existence of substrate-specific guilds for bacteria in temperate forest soils

  • Popis výsledku v původním jazyce

    Bacteria play critical roles in soil ecosystems when decomposing structural components of biomass. However, the ability of individual bacterial taxa to utilize various biopolymers is understudied, hampering our understanding of the role of bacteria in the soil carbon cycle. Here, we in situ incubated in forest litter various biopolymers of plant and fungal origin cellulose, xylan, glucomannan, pectin, lignin, 0-1,3-glucan, 0-1,3-1,6-glucan, and chitin to identify bacteria that associated with them during decomposition. After an incubation time of three weeks, all biopolymers were colonized by substantial bacterial numbers. The bacterial communities established on each biopolymer were specific, differing from the community on the surrounding plant litter, which indicates specialization in biopolymer utilization and the existence of distinct substrate-specific guilds. Members of Proteobacteria and Bacteroidetes predominated in all guilds. However, several biopolymers hosted members of other phyla: bacteria affiliated with Planctomycetes were enriched on cellulose, Acidobacteria on xylan, Actinobacteria on pectin, and Firmicutes on glucomannan and 0-1,3-1,6-glucan. The communities on lignin had low diversity, were phylogenetically clustered and were mainly composed of Proteobacteria. The communities on chitin showed higher diversity than those on other biopolymers. Approximately 80% of biopolymer-associated bacteria were specialists and were recovered from only one or two biopolymers. Only three specific phylotypes affiliated with Burkholderia, Klebsiella and Hafnia were present on all biopolymers. Bacterial isolation confirmed the involvement of Bacteroidetes in the decomposition of chitin, Firmicutes in the decomposition of glucomannan and 0-glucans, and the abundance of decomposers from Proteobacteria on all biopolymers. The proliferation of bacteria was observed on all fungally derived biopolymers and most plant-derived biopolymers. Exceptions were pectin and xylan, where bacterial counts were low three orders of magnitude lower than in the surrounding plant litter. The results indicate the involvement of distinct, substrate-specific guilds of bacteria in the utilization of biopolymers in forest topsoil. Furthermore, by classifying soil bacteria into substrate-specific guilds, this paper contributes to efforts to assign functional traits of ecological relevance to individual members of the microbial community.

  • Název v anglickém jazyce

    Specific utilization of biopolymers of plant and fungal origin reveals the existence of substrate-specific guilds for bacteria in temperate forest soils

  • Popis výsledku anglicky

    Bacteria play critical roles in soil ecosystems when decomposing structural components of biomass. However, the ability of individual bacterial taxa to utilize various biopolymers is understudied, hampering our understanding of the role of bacteria in the soil carbon cycle. Here, we in situ incubated in forest litter various biopolymers of plant and fungal origin cellulose, xylan, glucomannan, pectin, lignin, 0-1,3-glucan, 0-1,3-1,6-glucan, and chitin to identify bacteria that associated with them during decomposition. After an incubation time of three weeks, all biopolymers were colonized by substantial bacterial numbers. The bacterial communities established on each biopolymer were specific, differing from the community on the surrounding plant litter, which indicates specialization in biopolymer utilization and the existence of distinct substrate-specific guilds. Members of Proteobacteria and Bacteroidetes predominated in all guilds. However, several biopolymers hosted members of other phyla: bacteria affiliated with Planctomycetes were enriched on cellulose, Acidobacteria on xylan, Actinobacteria on pectin, and Firmicutes on glucomannan and 0-1,3-1,6-glucan. The communities on lignin had low diversity, were phylogenetically clustered and were mainly composed of Proteobacteria. The communities on chitin showed higher diversity than those on other biopolymers. Approximately 80% of biopolymer-associated bacteria were specialists and were recovered from only one or two biopolymers. Only three specific phylotypes affiliated with Burkholderia, Klebsiella and Hafnia were present on all biopolymers. Bacterial isolation confirmed the involvement of Bacteroidetes in the decomposition of chitin, Firmicutes in the decomposition of glucomannan and 0-glucans, and the abundance of decomposers from Proteobacteria on all biopolymers. The proliferation of bacteria was observed on all fungally derived biopolymers and most plant-derived biopolymers. Exceptions were pectin and xylan, where bacterial counts were low three orders of magnitude lower than in the surrounding plant litter. The results indicate the involvement of distinct, substrate-specific guilds of bacteria in the utilization of biopolymers in forest topsoil. Furthermore, by classifying soil bacteria into substrate-specific guilds, this paper contributes to efforts to assign functional traits of ecological relevance to individual members of the microbial community.

Klasifikace

  • Druh

    J<sub>imp</sub> - Článek v periodiku v databázi Web of Science

  • CEP obor

  • OECD FORD obor

    10606 - Microbiology

Návaznosti výsledku

  • Projekt

    Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

  • Návaznosti

    I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Ostatní

  • Rok uplatnění

    2022

  • Kód důvěrnosti údajů

    S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Údaje specifické pro druh výsledku

  • Název periodika

    Soil Biology and Biochemistry

  • ISSN

    0038-0717

  • e-ISSN

  • Svazek periodika

    171

  • Číslo periodika v rámci svazku

    AUG 2022

  • Stát vydavatele periodika

    GB - Spojené království Velké Británie a Severního Irska

  • Počet stran výsledku

    10

  • Strana od-do

    108696

  • Kód UT WoS článku

    000808182500006

  • EID výsledku v databázi Scopus

    2-s2.0-85130524427