Taxonomy of Aspergillus series Versicolores: species reduction and lessons learned about intraspecific variability

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388971%3A_____%2F22%3A00566493" target="_blank" >RIV/61388971:_____/22:00566493 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/00216208:11310/22:10457320

Výsledek na webu

<a href="https://www.studiesinmycology.org/index.php/issue/116-studies-in-mycology-no-102" target="_blank" >https://www.studiesinmycology.org/index.php/issue/116-studies-in-mycology-no-102</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.3114/sim.2022.102.02" target="_blank" >10.3114/sim.2022.102.02</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Taxonomy of Aspergillus series Versicolores: species reduction and lessons learned about intraspecific variability

Popis výsledku v původním jazyce

Aspergillus series Versicolores members occur in a wide range of environments and substrates such as indoor environments, food, clinical materials, soil, caves, marine or hypersaline ecosystems. The taxonomy of the series has undergone numerous re-arrangements including a drastic reduction in the number of species and subsequent recovery to 17 species in the last decade. The identification to species level is however problematic or impossible in some isolates even using DNA sequencing or MALDI-TOF mass spectrometry indicating a problem in the definition of species boundaries. To revise the species limits, we assembled a large dataset of 518 strains. From these, a total of 213 strains were selected for the final analysis according to their calmodulin (CaM) genotype, substrate and geography. This set was used for phylogenetic analysis based on five loci (benA, CaM, RPB2, Mcm7, Tsr1). Apart from the classical phylogenetic methods, we used multispecies coalescence (MSC) model-based methods, including one multilocus method (STACEY) and five single-locus methods (GMYC, bGMYC, PTP, bPTP, ABGD). Almost all species delimitation methods suggested a broad species concept with only four species consistentlynsupported. We also demonstrated that the currently applied concept of species is not sustainable as there are incongruences between single-gene phylogenies resulting in different species identifications when using different gene regions. Morphological and physiological data showed overall lack of good, taxonomically informative characters, which could be used for identification of such a large number of existing species. The characters expressed either low variability across species or significant intraspecific variability exceeding interspecific variability.

Název v anglickém jazyce

Taxonomy of Aspergillus series Versicolores: species reduction and lessons learned about intraspecific variability

Popis výsledku anglicky

Aspergillus series Versicolores members occur in a wide range of environments and substrates such as indoor environments, food, clinical materials, soil, caves, marine or hypersaline ecosystems. The taxonomy of the series has undergone numerous re-arrangements including a drastic reduction in the number of species and subsequent recovery to 17 species in the last decade. The identification to species level is however problematic or impossible in some isolates even using DNA sequencing or MALDI-TOF mass spectrometry indicating a problem in the definition of species boundaries. To revise the species limits, we assembled a large dataset of 518 strains. From these, a total of 213 strains were selected for the final analysis according to their calmodulin (CaM) genotype, substrate and geography. This set was used for phylogenetic analysis based on five loci (benA, CaM, RPB2, Mcm7, Tsr1). Apart from the classical phylogenetic methods, we used multispecies coalescence (MSC) model-based methods, including one multilocus method (STACEY) and five single-locus methods (GMYC, bGMYC, PTP, bPTP, ABGD). Almost all species delimitation methods suggested a broad species concept with only four species consistentlynsupported. We also demonstrated that the currently applied concept of species is not sustainable as there are incongruences between single-gene phylogenies resulting in different species identifications when using different gene regions. Morphological and physiological data showed overall lack of good, taxonomically informative characters, which could be used for identification of such a large number of existing species. The characters expressed either low variability across species or significant intraspecific variability exceeding interspecific variability.

Druh

J_ost - Ostatní články v recenzovaných periodicích

CEP obor

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OECD FORD obor

10606 - Microbiology

Projekt

<a href="/cs/project/NU21-05-00681" target="_blank" >NU21-05-00681: Důsledky hybridizace pro šíření antifungálních rezistencí a vymezení druhů u patogenních hub: modelová studie u rodu Aspergillus a Trichophyton</a><br>

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2022

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Studies in Mycology

ISSN

0166-0616

e-ISSN

1872-9797

Svazek periodika

102

Číslo periodika v rámci svazku

November 16

Stát vydavatele periodika

NL - Nizozemsko

Počet stran výsledku

41

Strana od-do

53-93

Kód UT WoS článku

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EID výsledku v databázi Scopus

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