In vitro interactions of TiO2 Nanoparticles with earthworm coelomocytes: immunotoxicity assessment. Nanomaterials

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388971%3A_____%2F22%3A00572819" target="_blank" >RIV/61388971:_____/22:00572819 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/61388971:_____/22:00559249

Výsledek na webu

<a href="https://www.mdpi.com/2079-4991/11/1/250" target="_blank" >https://www.mdpi.com/2079-4991/11/1/250</a>

DOI - Digital Object Identifier

—

Jazyk výsledku

angličtina

Název v původním jazyce

In vitro interactions of TiO2 Nanoparticles with earthworm coelomocytes: immunotoxicity assessment. Nanomaterials

Popis výsledku v původním jazyce

Titanium dioxide nanoparticles (TiO2 NPs) are manufactured worldwide. Once they arrivein the soil environment, they can endanger living organisms. Hence, monitoring and assessing theeffects of these nanoparticles is required. We focus on the Eisenia andrei earthworm immune cellsexposed to sublethal concentrations of TiO2 NPs (1, 10, and 100 μg/mL) for 2, 6, and 24 h. TiO2 NPsnat all concentrations did not affect cell viability. Further, TiO2 NPs did not cause changes in reactiveoxygen species (ROS) production, malondialdehyde (MDA) production, and phagocytic activity.Similarly, they did not elicit DNA damage. Overall, we did not detect any toxic effects of TiO2 NPsat the cellular level. At the gene expression level, slight changes were detected. Metallothionein,fetidin/lysenin, lumbricin and MEK kinase I were upregulated in coelomocytes after exposure to10 μg/mL TiO2 NPs for 6 h. Antioxidant enzyme expression was similar in exposed and control cells.TiO2 NPs were detected on coelomocyte membranes. However, our results do not show any strongeffects of these nanoparticles on coelomocytes at both the cellular and molecular levels.n

Název v anglickém jazyce

In vitro interactions of TiO2 Nanoparticles with earthworm coelomocytes: immunotoxicity assessment. Nanomaterials

Popis výsledku anglicky

Titanium dioxide nanoparticles (TiO2 NPs) are manufactured worldwide. Once they arrivein the soil environment, they can endanger living organisms. Hence, monitoring and assessing theeffects of these nanoparticles is required. We focus on the Eisenia andrei earthworm immune cellsexposed to sublethal concentrations of TiO2 NPs (1, 10, and 100 μg/mL) for 2, 6, and 24 h. TiO2 NPsnat all concentrations did not affect cell viability. Further, TiO2 NPs did not cause changes in reactiveoxygen species (ROS) production, malondialdehyde (MDA) production, and phagocytic activity.Similarly, they did not elicit DNA damage. Overall, we did not detect any toxic effects of TiO2 NPsat the cellular level. At the gene expression level, slight changes were detected. Metallothionein,fetidin/lysenin, lumbricin and MEK kinase I were upregulated in coelomocytes after exposure to10 μg/mL TiO2 NPs for 6 h. Antioxidant enzyme expression was similar in exposed and control cells.TiO2 NPs were detected on coelomocyte membranes. However, our results do not show any strongeffects of these nanoparticles on coelomocytes at both the cellular and molecular levels.n

Druh

C - Kapitola v odborné knize

CEP obor

—

OECD FORD obor

30102 - Immunology

Projekt

<a href="/cs/project/QK1910095" target="_blank" >QK1910095: Využití vermikompostování k eliminaci mikropolutantů za účelem bezpečné aplikace čistírenského kalu na zemědělskou půdu</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2022

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název knihy nebo sborníku

An Evolutionary and Environmental Perspective of the Interaction of Nanomaterials with the Immune System-The Outcomes of the EU Project PANDORA

ISBN

978-3-0365-3948-5

Počet stran výsledku

17

Strana od-do

49-65

Počet stran knihy

218

Název nakladatele

MDPI

Místo vydání

Basel

Kód UT WoS kapitoly

—