Non-target biotransformation enzymes as a target for triazole-zinc mixtures

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388971%3A_____%2F23%3A00574688" target="_blank" >RIV/61388971:_____/23:00574688 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/60460709:41210/23:94804 RIV/00216208:11310/23:10465865 RIV/00216208:11510/23:10465865

Výsledek na webu

<a href="https://www.sciencedirect.com/science/article/pii/S0009279723002922?via%3Dihub" target="_blank" >https://www.sciencedirect.com/science/article/pii/S0009279723002922?via%3Dihub</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.cbi.2023.110625" target="_blank" >10.1016/j.cbi.2023.110625</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Non-target biotransformation enzymes as a target for triazole-zinc mixtures

Popis výsledku v původním jazyce

Triazoles inhibit lanosterol 14 & alpha,-demethylase and block ergosterol biosynthesis in fungal pathogens. However, they also interact with other cytochrome P450 enzymes and influence non-target metabolic pathways. Disturbingly, triazoles may interact with essential elements. The interaction of penconazole (Pen), cyproconazole (Cyp) and tebuconazole (Teb) with Zn2+ results in the formation of deprotonated ligands in their complexes or in the creation of complexes with Cl- as a counterion or doubly charged complexes. Triazoles, as well as their equimolar cocktails with Zn2+ (10-6 mol/L), decreased the activities of the non-target enzymes CYP19A1 and CYP3A4. Pen most decreased CYP19A1 activity and was best bound to its active centre to block the catalytic cycle in computational analysis. For CYP3A4, Teb was found to be the most effective inhibitor by both, activity assay and interaction with the active centre. Teb/Cyp/Zn2+ and Teb/Pen/Cyp/Zn2+ cocktails also decreased the CYP19A1 activity, which was in correlation with the formation of numerous triazole-Zn2+ complexes.

Název v anglickém jazyce

Non-target biotransformation enzymes as a target for triazole-zinc mixtures

Popis výsledku anglicky

Triazoles inhibit lanosterol 14 & alpha,-demethylase and block ergosterol biosynthesis in fungal pathogens. However, they also interact with other cytochrome P450 enzymes and influence non-target metabolic pathways. Disturbingly, triazoles may interact with essential elements. The interaction of penconazole (Pen), cyproconazole (Cyp) and tebuconazole (Teb) with Zn2+ results in the formation of deprotonated ligands in their complexes or in the creation of complexes with Cl- as a counterion or doubly charged complexes. Triazoles, as well as their equimolar cocktails with Zn2+ (10-6 mol/L), decreased the activities of the non-target enzymes CYP19A1 and CYP3A4. Pen most decreased CYP19A1 activity and was best bound to its active centre to block the catalytic cycle in computational analysis. For CYP3A4, Teb was found to be the most effective inhibitor by both, activity assay and interaction with the active centre. Teb/Cyp/Zn2+ and Teb/Pen/Cyp/Zn2+ cocktails also decreased the CYP19A1 activity, which was in correlation with the formation of numerous triazole-Zn2+ complexes.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10606 - Microbiology

Projekt

—

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2023

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Chemico-Biological Interactions

ISSN

0009-2797

e-ISSN

1872-7786

Svazek periodika

382

Číslo periodika v rámci svazku

1 September

Stát vydavatele periodika

IE - Irsko

Počet stran výsledku

8

Strana od-do

110625

Kód UT WoS článku

001039350600001

EID výsledku v databázi Scopus

2-s2.0-85164817854