Antibiotic resistance and biofilm-forming ability of a-toxin-positive Clostridium septicum isolates worsen patient prognosis

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388971%3A_____%2F23%3A00576018" target="_blank" >RIV/61388971:_____/23:00576018 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/00216208:11310/23:10477281

Výsledek na webu

<a href="https://onlinelibrary.wiley.com/doi/10.1111/apm.13338" target="_blank" >https://onlinelibrary.wiley.com/doi/10.1111/apm.13338</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1111/apm.13338" target="_blank" >10.1111/apm.13338</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Antibiotic resistance and biofilm-forming ability of a-toxin-positive Clostridium septicum isolates worsen patient prognosis

Popis výsledku v původním jazyce

A total of, 78 Clostridium septicum (CLSE) isolates were screened for genes encoding: a-toxin, flagellin, and resistance to vancomycin (VANg). The isolates were also tested for their ability to form biofilm and their antibiotic susceptibility. All isolates were positive for a-toxin and flagellin genes. However, only 19 isolates (24.3%) showed prevalence for VANg. We observed the strongest capacity to form a biofilm (100%) in isolates from patients with oncologic or septic and febrile diagnoses. This percentage was also very high in patients with colitis and gastrointestinal hemorrhage (72.7%). No less than 43 isolates showed antibiotic resistance, and 21 were multidrug-resistant (MDR). Interestingly, our studies showed a correlation between antibiotic resistance and biofilm formation. A statistically significant difference was observed between biofilm-forming MDR isolates and those with low/no biofilm-forming ability. However, the most impressive observation was the correlation with mortality rate. While the overall mortality rate for CLSE infections was 16.7% (13/78), the mortality rate for patients infected with MDR isolates forming biofilm moderately or strongly reached 38.1% (8/21). This number increased even further when only infections with the biofilm-forming VANg-positive isolates were considered (61.5%, 8/13). Therefore, the ability of a VANg-positive CLSE isolate to form a biofilm has been suggested as a biomarker of poor prognosis.

Název v anglickém jazyce

Antibiotic resistance and biofilm-forming ability of a-toxin-positive Clostridium septicum isolates worsen patient prognosis

Popis výsledku anglicky

A total of, 78 Clostridium septicum (CLSE) isolates were screened for genes encoding: a-toxin, flagellin, and resistance to vancomycin (VANg). The isolates were also tested for their ability to form biofilm and their antibiotic susceptibility. All isolates were positive for a-toxin and flagellin genes. However, only 19 isolates (24.3%) showed prevalence for VANg. We observed the strongest capacity to form a biofilm (100%) in isolates from patients with oncologic or septic and febrile diagnoses. This percentage was also very high in patients with colitis and gastrointestinal hemorrhage (72.7%). No less than 43 isolates showed antibiotic resistance, and 21 were multidrug-resistant (MDR). Interestingly, our studies showed a correlation between antibiotic resistance and biofilm formation. A statistically significant difference was observed between biofilm-forming MDR isolates and those with low/no biofilm-forming ability. However, the most impressive observation was the correlation with mortality rate. While the overall mortality rate for CLSE infections was 16.7% (13/78), the mortality rate for patients infected with MDR isolates forming biofilm moderately or strongly reached 38.1% (8/21). This number increased even further when only infections with the biofilm-forming VANg-positive isolates were considered (61.5%, 8/13). Therefore, the ability of a VANg-positive CLSE isolate to form a biofilm has been suggested as a biomarker of poor prognosis.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10606 - Microbiology

Projekt

—

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2023

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Acta Pathologica Microbiologica et Immunologica Scandinavica

ISSN

0903-4641

e-ISSN

1600-0463

Svazek periodika

131

Číslo periodika v rámci svazku

8

Stát vydavatele periodika

DK - Dánské království

Počet stran výsledku

8

Strana od-do

434-441

Kód UT WoS článku

001019829100001

EID výsledku v databázi Scopus

2-s2.0-85162856576