Dimerisation of the Yeast K+Translocation Protein Trk1 Depends on the K+Concentration
Identifikátory výsledku
Kód výsledku v IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388971%3A_____%2F23%3A00578992" target="_blank" >RIV/61388971:_____/23:00578992 - isvavai.cz</a>
Výsledek na webu
<a href="https://www.mdpi.com/1422-0067/24/1/398" target="_blank" >https://www.mdpi.com/1422-0067/24/1/398</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.3390/ijms24010398" target="_blank" >10.3390/ijms24010398</a>
Alternativní jazyky
Jazyk výsledku
angličtina
Název v původním jazyce
Dimerisation of the Yeast K+Translocation Protein Trk1 Depends on the K+Concentration
Popis výsledku v původním jazyce
In baker's yeast (Saccharomyces cerevisiae), Trk1, a member of the superfamily of K-transporters (SKT), is the main K+ uptake system under conditions when its concentration in the environment is low. Structurally, Trk1 is made up of four domains, each similar and homologous to a K-channel alpha subunit. Because most K-channels are proteins containing four channel-building alpha subunits, Trk1 could be functional as a monomer. However, related SKT proteins TrkH and KtrB were crystallised as dimers, and for Trk1, a tetrameric arrangement has been proposed based on molecular modelling. Here, based on Bimolecular Fluorescence Complementation experiments and single-molecule fluorescence microscopy combined with molecular modelling, we provide evidence that Trk1 can exist in the yeast plasma membrane as a monomer as well as a dimer. The association of monomers to dimers is regulated by the K+ concentration.
Název v anglickém jazyce
Dimerisation of the Yeast K+Translocation Protein Trk1 Depends on the K+Concentration
Popis výsledku anglicky
In baker's yeast (Saccharomyces cerevisiae), Trk1, a member of the superfamily of K-transporters (SKT), is the main K+ uptake system under conditions when its concentration in the environment is low. Structurally, Trk1 is made up of four domains, each similar and homologous to a K-channel alpha subunit. Because most K-channels are proteins containing four channel-building alpha subunits, Trk1 could be functional as a monomer. However, related SKT proteins TrkH and KtrB were crystallised as dimers, and for Trk1, a tetrameric arrangement has been proposed based on molecular modelling. Here, based on Bimolecular Fluorescence Complementation experiments and single-molecule fluorescence microscopy combined with molecular modelling, we provide evidence that Trk1 can exist in the yeast plasma membrane as a monomer as well as a dimer. The association of monomers to dimers is regulated by the K+ concentration.
Klasifikace
Druh
J<sub>imp</sub> - Článek v periodiku v databázi Web of Science
CEP obor
—
OECD FORD obor
10606 - Microbiology
Návaznosti výsledku
Projekt
Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.
Návaznosti
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Ostatní
Rok uplatnění
2023
Kód důvěrnosti údajů
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Údaje specifické pro druh výsledku
Název periodika
International Journal of Molecular Sciences
ISSN
1422-0067
e-ISSN
1422-0067
Svazek periodika
24
Číslo periodika v rámci svazku
1
Stát vydavatele periodika
CH - Švýcarská konfederace
Počet stran výsledku
16
Strana od-do
398
Kód UT WoS článku
000909320300001
EID výsledku v databázi Scopus
2-s2.0-85145987316