Microdomains heterogeneity in the thylakoid membrane proteins visualized by super-resolution microscopy

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388971%3A_____%2F23%3A00580758" target="_blank" >RIV/61388971:_____/23:00580758 - isvavai.cz</a>

Výsledek na webu

<a href="https://ps.ueb.cas.cz/artkey/phs-202304-0009_microdomains-heterogeneity-in-the-thylakoid-membrane-proteins-visualized-by-super-resolution-microscopy.php" target="_blank" >https://ps.ueb.cas.cz/artkey/phs-202304-0009_microdomains-heterogeneity-in-the-thylakoid-membrane-proteins-visualized-by-super-resolution-microscopy.php</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.32615/ps.2023.043" target="_blank" >10.32615/ps.2023.043</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Microdomains heterogeneity in the thylakoid membrane proteins visualized by super-resolution microscopy

Popis výsledku v původním jazyce

The investigation of spatial heterogeneity within the thylakoid membrane (TM) proteins has gained increasing attention in photosynthetic research. The recent advances in live-cell imaging have allowed the identification of heterogeneous organisation of photosystems in small cyanobacterial cells. These sub-micrometre TM regions, termed microdomains in cyanobacteria, exhibit functional similarities with granal (Photosystem II dominant) and stromal (Photosystem I dominant) regions observed in TM of higher plants. This study delves into microdomain heterogeneity using superresolution Airyscan-based microscopy enhancing resolution to approximately ~125 nm in x-y dimension. The new data reveal membrane areas rich in Photosystem I within the inner TM rings. Moreover, we identified analogous dynamics in the mobility of Photosystem II and phycobilisomes, countering earlier models that postulated differing mobility of these complexes. These novel findings thus hold significance for our understanding of photosynthesis regulation, particularly during state transitions.

Název v anglickém jazyce

Microdomains heterogeneity in the thylakoid membrane proteins visualized by super-resolution microscopy

Popis výsledku anglicky

The investigation of spatial heterogeneity within the thylakoid membrane (TM) proteins has gained increasing attention in photosynthetic research. The recent advances in live-cell imaging have allowed the identification of heterogeneous organisation of photosystems in small cyanobacterial cells. These sub-micrometre TM regions, termed microdomains in cyanobacteria, exhibit functional similarities with granal (Photosystem II dominant) and stromal (Photosystem I dominant) regions observed in TM of higher plants. This study delves into microdomain heterogeneity using superresolution Airyscan-based microscopy enhancing resolution to approximately ~125 nm in x-y dimension. The new data reveal membrane areas rich in Photosystem I within the inner TM rings. Moreover, we identified analogous dynamics in the mobility of Photosystem II and phycobilisomes, countering earlier models that postulated differing mobility of these complexes. These novel findings thus hold significance for our understanding of photosynthesis regulation, particularly during state transitions.

Druh

J_SC - Článek v periodiku v databázi SCOPUS

CEP obor

—

OECD FORD obor

10606 - Microbiology

Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2023

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Photosynthetica

ISSN

0300-3604

e-ISSN

1573-9058

Svazek periodika

61

Číslo periodika v rámci svazku

Special issue

Stát vydavatele periodika

CZ - Česká republika

Počet stran výsledku

9

Strana od-do

483-491

Kód UT WoS článku

001128608100001

EID výsledku v databázi Scopus

2-s2.0-85180856825