Phylogenetically related soil actinomycetes distinguish isolation sites by their metabolic activities

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388971%3A_____%2F23%3A00581040" target="_blank" >RIV/61388971:_____/23:00581040 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/60460709:41210/23:96470 RIV/00216208:11320/23:10472879 RIV/00027006:_____/23:10176619

Výsledek na webu

<a href="https://academic.oup.com/femsec/article/99/12/fiad139/7342452?login=true" target="_blank" >https://academic.oup.com/femsec/article/99/12/fiad139/7342452?login=true</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1093/femsec/fiad139" target="_blank" >10.1093/femsec/fiad139</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Phylogenetically related soil actinomycetes distinguish isolation sites by their metabolic activities

Popis výsledku v původním jazyce

Soil environments are inhabited by microorganisms adapted to its diversified microhabitats. The metabolic activity of individual strains/populations reflects resources available at a particular spot, quality of which may not comply with broad soil characteristics. To explore the potential of individual strains to adapt to particular micro-niches of carbon sources, a set of 331 Actinomycetia strains were collected at ten sites differing in vegetation, soil pH, organic matter content and quality. The strains were isolated on the same complex medium with neutral pH and their metabolites analyzed by UHPLC and LC-MS/MS in spent cultivation medium (metabolic profiles). For all strains, their metabolic profiles correlated with soil pH and organic matter content of the original sites. In comparison, strains phylogeny based on either 16S rRNA or the beta-subunit of DNA-dependent RNA polymerase (rpoB) genes was partially correlated with soil organic matter content but not soil pH at the sites. Antimicrobial activities of strains against Kocuria rhizophila, Escherichia coli, and Saccharomyces cerevisiae were both site- and phylogeny-dependent. The precise adaptation of metabolic profiles to overall sites characteristics was further supported by the production of locally specific bioactive metabolites and suggested that carbon resources represent a significant selection pressure connected to specific antibiotic activities.

Název v anglickém jazyce

Phylogenetically related soil actinomycetes distinguish isolation sites by their metabolic activities

Popis výsledku anglicky

Soil environments are inhabited by microorganisms adapted to its diversified microhabitats. The metabolic activity of individual strains/populations reflects resources available at a particular spot, quality of which may not comply with broad soil characteristics. To explore the potential of individual strains to adapt to particular micro-niches of carbon sources, a set of 331 Actinomycetia strains were collected at ten sites differing in vegetation, soil pH, organic matter content and quality. The strains were isolated on the same complex medium with neutral pH and their metabolites analyzed by UHPLC and LC-MS/MS in spent cultivation medium (metabolic profiles). For all strains, their metabolic profiles correlated with soil pH and organic matter content of the original sites. In comparison, strains phylogeny based on either 16S rRNA or the beta-subunit of DNA-dependent RNA polymerase (rpoB) genes was partially correlated with soil organic matter content but not soil pH at the sites. Antimicrobial activities of strains against Kocuria rhizophila, Escherichia coli, and Saccharomyces cerevisiae were both site- and phylogeny-dependent. The precise adaptation of metabolic profiles to overall sites characteristics was further supported by the production of locally specific bioactive metabolites and suggested that carbon resources represent a significant selection pressure connected to specific antibiotic activities.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10606 - Microbiology

Projekt

<a href="/cs/project/LTC20063" target="_blank" >LTC20063: Food-web interactions in relation to water regimes and soil pH during decomposition in the litter layer.</a><br>

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2023

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

FEMS Microbiology Ecology

ISSN

0168-6496

e-ISSN

1574-6941

Svazek periodika

99

Číslo periodika v rámci svazku

12

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

12

Strana od-do

fiad139

Kód UT WoS článku

001104177000003

EID výsledku v databázi Scopus

2-s2.0-85178478314