RIP-seq reveals RNAs that interact with RNA polymerase and primary sigma factors in bacteria

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388971%3A_____%2F24%3A00582831" target="_blank" >RIV/61388971:_____/24:00582831 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/60162694:G33__/24:N0000003 RIV/00216224:90242/24:00139162 RIV/00216208:11130/24:10477339 RIV/00216208:11310/24:10477339

Výsledek na webu

<a href="https://academic.oup.com/nar/advance-article/doi/10.1093/nar/gkae081/7606967?login=true" target="_blank" >https://academic.oup.com/nar/advance-article/doi/10.1093/nar/gkae081/7606967?login=true</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1093/nar/gkae081" target="_blank" >10.1093/nar/gkae081</a>

Jazyk výsledku

angličtina

Název v původním jazyce

RIP-seq reveals RNAs that interact with RNA polymerase and primary sigma factors in bacteria

Popis výsledku v původním jazyce

Bacteria have evolved structured RNAs that can associate with RNA polymerase (RNAP). Two of them have been known so far-6S RNA and Ms1 RNA but it is unclear if any other types of RNAs binding to RNAP exist in bacteria. To identify all RNAs interacting with RNAP and the primary σ factors, we have established and performed native RIP-seq in Bacillus subtilis, Corynebacterium glutamicum, Streptomyces coelicolor, Mycobacterium smegmatis and the pathogenic Mycobacterium tuberculosis. Besides known 6S RNAs in B. subtilis and Ms1 in M. smegmatis, we detected MTS2823, a homologue of Ms1, on RNAP in M. tuberculosis. In C. glutamicum, we discovered novel types of structured RNAs that associate with RNAP. Furthermore, we identified other species-specific RNAs including full-length mRNAs, revealing a previously unknown landscape of RNAs interacting with the bacterial transcription machinery.

Název v anglickém jazyce

RIP-seq reveals RNAs that interact with RNA polymerase and primary sigma factors in bacteria

Popis výsledku anglicky

Bacteria have evolved structured RNAs that can associate with RNA polymerase (RNAP). Two of them have been known so far-6S RNA and Ms1 RNA but it is unclear if any other types of RNAs binding to RNAP exist in bacteria. To identify all RNAs interacting with RNAP and the primary σ factors, we have established and performed native RIP-seq in Bacillus subtilis, Corynebacterium glutamicum, Streptomyces coelicolor, Mycobacterium smegmatis and the pathogenic Mycobacterium tuberculosis. Besides known 6S RNAs in B. subtilis and Ms1 in M. smegmatis, we detected MTS2823, a homologue of Ms1, on RNAP in M. tuberculosis. In C. glutamicum, we discovered novel types of structured RNAs that associate with RNAP. Furthermore, we identified other species-specific RNAs including full-length mRNAs, revealing a previously unknown landscape of RNAs interacting with the bacterial transcription machinery.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

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OECD FORD obor

10606 - Microbiology

Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2024

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Nucleic Acids Research

ISSN

0305-1048

e-ISSN

1362-4962

Svazek periodika

52

Číslo periodika v rámci svazku

May 8

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

23

Strana od-do

4604-4626

Kód UT WoS článku

001160557000001

EID výsledku v databázi Scopus

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