Accumulation and toxicity of biologically produced gold nanoparticles in different types of specialized mammalian cells

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388971%3A_____%2F24%3A00584751" target="_blank" >RIV/61388971:_____/24:00584751 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/86652036:_____/24:00584751 RIV/68081715:_____/24:00584751 RIV/46747885:24530/24:00012327

Výsledek na webu

<a href="https://iubmb.onlinelibrary.wiley.com/doi/10.1002/bab.2575" target="_blank" >https://iubmb.onlinelibrary.wiley.com/doi/10.1002/bab.2575</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1002/bab.2575" target="_blank" >10.1002/bab.2575</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Accumulation and toxicity of biologically produced gold nanoparticles in different types of specialized mammalian cells

Popis výsledku v původním jazyce

The biologically produced gold nanoparticles (AuNPs) are novel carriers with promising use in targeted tumor therapy. Still, there are no studies regarding the efficacy of nanoparticle internalization by cancer and noncancer cells. In this study, AuNPs were produced by Fusarium oxysporum and analyzed by spectrophotometry, transmission electron microscopy (TEM), energy dispersive x-ray spectroscopy (EDS), and Zetasizer. Obtained AuNPs were about 15 nm in size with a zeta potential of35.8 mV. The AuNPs were added to cancer cells (4T1), noncancer cells (NIH/3T3), and macrophages (RAW264.7). The viability decreased in 4T1 (77 +/- 3.74%) in contrast to NIH/3T3 and RAW264.7 cells (89 +/- 4.9% and 90 +/- 3.5%, respectively). The 4T1 cancer cells also showed the highest uptake and accumulation of Au (similar to 80% of AuNPs was internalized) as determined by graphite furnace atomic absorption spectroscopy. The lowest amount of AuNPs was internalized by the NIH/3T3 cells (similar to 30%). The NIH/3T3 cells exhibited prominent reorganization of F-actin filaments as examined by confocal microscopy. In RAW264.7, we analyzed the release of proinflammatory cytokines by flow cytometry and we found the AuNP interaction triggered transient secretion of tumor necrosis factor alpha (TNF-alpha) and interferon gamma (IFN-gamma). In summary, we proved the biologically produced AuNPs entered all the tested cell types and triggered cell-specific responses. High AuNP uptake by tumor cells was related to decreased cell viability, while low nanoparticle uptake by fibroblasts triggered F-actin reorganization without remarkable toxicity. Thus, the biologically produced AuNPs hold promising potential as cancer drug carriers and likely require proper surface functionalization to shield phagocytizing cells.

Název v anglickém jazyce

Accumulation and toxicity of biologically produced gold nanoparticles in different types of specialized mammalian cells

Popis výsledku anglicky

The biologically produced gold nanoparticles (AuNPs) are novel carriers with promising use in targeted tumor therapy. Still, there are no studies regarding the efficacy of nanoparticle internalization by cancer and noncancer cells. In this study, AuNPs were produced by Fusarium oxysporum and analyzed by spectrophotometry, transmission electron microscopy (TEM), energy dispersive x-ray spectroscopy (EDS), and Zetasizer. Obtained AuNPs were about 15 nm in size with a zeta potential of35.8 mV. The AuNPs were added to cancer cells (4T1), noncancer cells (NIH/3T3), and macrophages (RAW264.7). The viability decreased in 4T1 (77 +/- 3.74%) in contrast to NIH/3T3 and RAW264.7 cells (89 +/- 4.9% and 90 +/- 3.5%, respectively). The 4T1 cancer cells also showed the highest uptake and accumulation of Au (similar to 80% of AuNPs was internalized) as determined by graphite furnace atomic absorption spectroscopy. The lowest amount of AuNPs was internalized by the NIH/3T3 cells (similar to 30%). The NIH/3T3 cells exhibited prominent reorganization of F-actin filaments as examined by confocal microscopy. In RAW264.7, we analyzed the release of proinflammatory cytokines by flow cytometry and we found the AuNP interaction triggered transient secretion of tumor necrosis factor alpha (TNF-alpha) and interferon gamma (IFN-gamma). In summary, we proved the biologically produced AuNPs entered all the tested cell types and triggered cell-specific responses. High AuNP uptake by tumor cells was related to decreased cell viability, while low nanoparticle uptake by fibroblasts triggered F-actin reorganization without remarkable toxicity. Thus, the biologically produced AuNPs hold promising potential as cancer drug carriers and likely require proper surface functionalization to shield phagocytizing cells.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10606 - Microbiology

Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2024

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Biotechnology and Applied Biochemistry

ISSN

0885-4513

e-ISSN

1470-8744

Svazek periodika

71

Číslo periodika v rámci svazku

4

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

13

Strana od-do

766-778

Kód UT WoS článku

001184114000001

EID výsledku v databázi Scopus

2-s2.0-85188097182