Design Rules for the Nano-Bio Interface of Nanodiamonds: Implications for siRNA Vectorization

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388971%3A_____%2F24%3A00585159" target="_blank" >RIV/61388971:_____/24:00585159 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/61388963:_____/24:00585159 RIV/00216208:11310/24:10495579

Výsledek na webu

<a href="https://doi.org/10.1002/adfm.202314088" target="_blank" >https://doi.org/10.1002/adfm.202314088</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1002/adfm.202314088" target="_blank" >10.1002/adfm.202314088</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Design Rules for the Nano-Bio Interface of Nanodiamonds: Implications for siRNA Vectorization

Popis výsledku v původním jazyce

The enormous therapeutic potential of selective ribonucleic acid (RNA) interference has recently been manifested by the approval of several small interfering RNA (siRNA)-based drugs. However, the efficacy of siRNA delivery is still limited, and an extensive search for alternative and highly effective delivery approaches is ongoing. With this aim, three generations of non-viral vectors based on modified nanodiamonds (NDs) have been gradually developed in the past decade. They show great promise due to the negligible toxicity of the ND core. Here, a robust methodological approach is presented to enable the evaluation of new vector nanomaterials. Using a new type of third-generation ND vector coated with a copolymer with tunable charge density, variables such as colloidal stability, surface electrostatic properties, the molecular composition of the copolymer, and the mode of complexation with siRNA are optimized. Using an innovative data processing strategy, the results are related to biological potency, toxicity, and cell proliferation. Finally, the optimized composition of a coating copolymer consisting of a cationic component, 2-dimethylaminoethyl methacrylate, and an electroneutral biocompatible component, N-(2-hydroxypropyl) methacrylamide, is evaluated. The optimized NDs vectors are colloidally and biologically stable siRNA delivery tools with broad potential for RNA interference-based therapeutics.

Název v anglickém jazyce

Design Rules for the Nano-Bio Interface of Nanodiamonds: Implications for siRNA Vectorization

Popis výsledku anglicky

The enormous therapeutic potential of selective ribonucleic acid (RNA) interference has recently been manifested by the approval of several small interfering RNA (siRNA)-based drugs. However, the efficacy of siRNA delivery is still limited, and an extensive search for alternative and highly effective delivery approaches is ongoing. With this aim, three generations of non-viral vectors based on modified nanodiamonds (NDs) have been gradually developed in the past decade. They show great promise due to the negligible toxicity of the ND core. Here, a robust methodological approach is presented to enable the evaluation of new vector nanomaterials. Using a new type of third-generation ND vector coated with a copolymer with tunable charge density, variables such as colloidal stability, surface electrostatic properties, the molecular composition of the copolymer, and the mode of complexation with siRNA are optimized. Using an innovative data processing strategy, the results are related to biological potency, toxicity, and cell proliferation. Finally, the optimized composition of a coating copolymer consisting of a cationic component, 2-dimethylaminoethyl methacrylate, and an electroneutral biocompatible component, N-(2-hydroxypropyl) methacrylamide, is evaluated. The optimized NDs vectors are colloidally and biologically stable siRNA delivery tools with broad potential for RNA interference-based therapeutics.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

21001 - Nano-materials (production and properties)

Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2024

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Advanced Functional Materials

ISSN

1616-301X

e-ISSN

1616-3028

Svazek periodika

34

Číslo periodika v rámci svazku

30

Stát vydavatele periodika

DE - Spolková republika Německo

Počet stran výsledku

15

Strana od-do

2314088

Kód UT WoS článku

001188966200001

EID výsledku v databázi Scopus

2-s2.0-85188542010