Alternative production of fucoxanthin and PUFAs using Chlorochromonas danica and Hibberdia magna , unicellular chrysophytes with different trophic modes

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388971%3A_____%2F24%3A00600239" target="_blank" >RIV/61388971:_____/24:00600239 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/67985939:_____/24:00587940 RIV/00216208:11310/24:10489205

Výsledek na webu

<a href="https://www.sciencedirect.com/science/article/pii/S2211926424002091?via%3Dihub" target="_blank" >https://www.sciencedirect.com/science/article/pii/S2211926424002091?via%3Dihub</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.algal.2024.103597" target="_blank" >10.1016/j.algal.2024.103597</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Alternative production of fucoxanthin and PUFAs using Chlorochromonas danica and Hibberdia magna , unicellular chrysophytes with different trophic modes

Popis výsledku v původním jazyce

Ochrophyte microalgae attract attention from the applied phycology perspective, due to their ability to grow rapidly, engage variable trophic modes, and simultaneously produce high-value compounds such as xanthophyll carotenoids and polyunsaturated fatty acids. Unlike more often considered marine diatoms and haptophytes, unicellular chrysophytes may represent a reasonable freshwater alternative. In this work, we introduced two representatives: Chlorochromonas danica (Ochromonadales) and Hibberdia magna (Hibberdiales). We compared their ability to produce target compounds in mixotrophic and photoautotrophic modes, respectively. Both organisms had a similar temperature optima (1822 degrees C), but light demands were much higher in the photoautotrophic H. magna . This work is the first report of fucoxanthin content and productivity by C. danica , showing that the presence of light enhanced the content of fucoxanthin 4.5-fold compared with darkness. For fucoxanthin productivity in the mixotrophic batch cultured C. danica , the optimal initial glucose dose was 10 g L-1 . Culture medium supplemented with mineral nutrients resulted in an increase in biomass and fucoxanthin productivity of C. danica achieving the highest biomass productivity of 0.81 +/- 0.06 g L-1 d-1 . H. magna accumulated a maximum of 4.54 +/- 0.04 mg g-1 DW of fucoxanthin, which was slightly more than the maximum value for C. danica of 3.99 +/- 0.19 mg g-1 DW. However, due to the lower biomass productivity of H. magna , the maximal fucoxanthin productivities reached very similar values of 1.15 +/- 0.05 and 1.16 +/- 0.01 mg L d-1 in C. danica and H. magna , respectively. Both organisms had a relatively high fatty acid content, accounting for 17 % and 19 % of DW in C. danica and H. magna , respectively. H. magna had a higher level of polyunsaturated fatty acids, which were more diverse, longer, and more unsaturated. The potential for utilization of selected chrysophytes as producers in a multi-target biorefinery is discussed.

Název v anglickém jazyce

Alternative production of fucoxanthin and PUFAs using Chlorochromonas danica and Hibberdia magna , unicellular chrysophytes with different trophic modes

Popis výsledku anglicky

Ochrophyte microalgae attract attention from the applied phycology perspective, due to their ability to grow rapidly, engage variable trophic modes, and simultaneously produce high-value compounds such as xanthophyll carotenoids and polyunsaturated fatty acids. Unlike more often considered marine diatoms and haptophytes, unicellular chrysophytes may represent a reasonable freshwater alternative. In this work, we introduced two representatives: Chlorochromonas danica (Ochromonadales) and Hibberdia magna (Hibberdiales). We compared their ability to produce target compounds in mixotrophic and photoautotrophic modes, respectively. Both organisms had a similar temperature optima (1822 degrees C), but light demands were much higher in the photoautotrophic H. magna . This work is the first report of fucoxanthin content and productivity by C. danica , showing that the presence of light enhanced the content of fucoxanthin 4.5-fold compared with darkness. For fucoxanthin productivity in the mixotrophic batch cultured C. danica , the optimal initial glucose dose was 10 g L-1 . Culture medium supplemented with mineral nutrients resulted in an increase in biomass and fucoxanthin productivity of C. danica achieving the highest biomass productivity of 0.81 +/- 0.06 g L-1 d-1 . H. magna accumulated a maximum of 4.54 +/- 0.04 mg g-1 DW of fucoxanthin, which was slightly more than the maximum value for C. danica of 3.99 +/- 0.19 mg g-1 DW. However, due to the lower biomass productivity of H. magna , the maximal fucoxanthin productivities reached very similar values of 1.15 +/- 0.05 and 1.16 +/- 0.01 mg L d-1 in C. danica and H. magna , respectively. Both organisms had a relatively high fatty acid content, accounting for 17 % and 19 % of DW in C. danica and H. magna , respectively. H. magna had a higher level of polyunsaturated fatty acids, which were more diverse, longer, and more unsaturated. The potential for utilization of selected chrysophytes as producers in a multi-target biorefinery is discussed.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10606 - Microbiology

Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2024

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Algal Research-Biomass Biofuels and Bioproducts

ISSN

2211-9264

e-ISSN

2211-9264

Svazek periodika

82

Číslo periodika v rámci svazku

August 24

Stát vydavatele periodika

NL - Nizozemsko

Počet stran výsledku

12

Strana od-do

103597

Kód UT WoS článku

001268552100001

EID výsledku v databázi Scopus

2-s2.0-85197812614