Aldoxime dehydratases: production, immobilization, and use in multistep processes

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388971%3A_____%2F24%3A00601349" target="_blank" >RIV/61388971:_____/24:00601349 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/00216208:11310/24:10495725

Výsledek na webu

<a href="https://link.springer.com/article/10.1007/s00253-024-13272-6" target="_blank" >https://link.springer.com/article/10.1007/s00253-024-13272-6</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1007/s00253-024-13272-6" target="_blank" >10.1007/s00253-024-13272-6</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Aldoxime dehydratases: production, immobilization, and use in multistep processes

Popis výsledku v původním jazyce

The synthesis of nitriles is of utmost importance for preparative organic chemistry. The classical routes are often associated with disadvantages such as toxicity of the reagents and drastic conditions. The uses of enzymes like aldoxime dehydratases (Oxds) and hydroxynitrile lyases constitute attractive benign alternatives. In this review, we summarize the recent trends regarding Oxds. Thousands of oxd genes were sequenced but less than thirty Oxds were investigated on protein level. We give an overview of these Oxds, their sequence analysis, conditions required for their overexpression, and their purification and assays. We then focus on the use of Oxds especially in multistep reactions combining the chemical or chemoenzymatic synthesis of aldoximes from different starting materials with the enzymatic dehydration of aldoximes to nitriles, possibly followed by the hydration of nitriles to amides. Progress in Oxd immobilization is also highlighted. Based on data published mainly in the last 5years, we evaluate the industrial prospects of these enzyme processes in comparison with some other innovations in nitrile synthesis. KEY POINTS: Aldoxime dehydratases (Oxds) are promising for cyanide-free routes to nitriles A comprehensive overview of wet-lab explored Oxds is provided Recent trends include combining Oxds with other enzymes or chemical catalysts.

Název v anglickém jazyce

Aldoxime dehydratases: production, immobilization, and use in multistep processes

Popis výsledku anglicky

The synthesis of nitriles is of utmost importance for preparative organic chemistry. The classical routes are often associated with disadvantages such as toxicity of the reagents and drastic conditions. The uses of enzymes like aldoxime dehydratases (Oxds) and hydroxynitrile lyases constitute attractive benign alternatives. In this review, we summarize the recent trends regarding Oxds. Thousands of oxd genes were sequenced but less than thirty Oxds were investigated on protein level. We give an overview of these Oxds, their sequence analysis, conditions required for their overexpression, and their purification and assays. We then focus on the use of Oxds especially in multistep reactions combining the chemical or chemoenzymatic synthesis of aldoximes from different starting materials with the enzymatic dehydration of aldoximes to nitriles, possibly followed by the hydration of nitriles to amides. Progress in Oxd immobilization is also highlighted. Based on data published mainly in the last 5years, we evaluate the industrial prospects of these enzyme processes in comparison with some other innovations in nitrile synthesis. KEY POINTS: Aldoxime dehydratases (Oxds) are promising for cyanide-free routes to nitriles A comprehensive overview of wet-lab explored Oxds is provided Recent trends include combining Oxds with other enzymes or chemical catalysts.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10608 - Biochemistry and molecular biology

Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2024

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Applied Microbiology and Biotechnology

ISSN

0175-7598

e-ISSN

1432-0614

Svazek periodika

108

Číslo periodika v rámci svazku

1

Stát vydavatele periodika

DE - Spolková republika Německo

Počet stran výsledku

19

Strana od-do

518

Kód UT WoS článku

001355204400001

EID výsledku v databázi Scopus

2-s2.0-85209173862