Immunologically active cell wall polysaccharides of green microalga Dictyosphaerium chlorelloides (Chlorellacea)
Identifikátory výsledku
Kód výsledku v IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388971%3A_____%2F25%3A00605183" target="_blank" >RIV/61388971:_____/25:00605183 - isvavai.cz</a>
Nalezeny alternativní kódy
RIV/61388963:_____/25:00605183 RIV/60461373:22330/25:43930803 RIV/00216208:11130/25:10490449 RIV/00216208:11310/25:10490449 RIV/00064203:_____/25:10490449
Výsledek na webu
<a href="https://www.sciencedirect.com/science/article/pii/S0144861725000232?via%3Dihub" target="_blank" >https://www.sciencedirect.com/science/article/pii/S0144861725000232?via%3Dihub</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/j.carbpol.2025.123242" target="_blank" >10.1016/j.carbpol.2025.123242</a>
Alternativní jazyky
Jazyk výsledku
angličtina
Název v původním jazyce
Immunologically active cell wall polysaccharides of green microalga Dictyosphaerium chlorelloides (Chlorellacea)
Popis výsledku v původním jazyce
Dictyosphaerium chlorelloides is a green microalga from the Chlorella clade that produces highly viscous exocellular polysaccharides. The cell wall polysaccharides of this alga have not been studied in detail. In this article, water-soluble polysaccharides from D. chlorelloides biomass were extracted with hot water and purified by preparative chromatography. The composition, structural features and molecular masses of subsequently eluted fractions F1, F2, F3, F4 and F5 (minor) were determined. Three high-yield products F1, F3 and F4 consisted mainly of galactopyranosyl, 2-O-methyl-galactopyranosyl, rhamnopyranosyl and mannopyranosyl units at different proportions, while F2 was rich in glucose. Immunoactivity of these fractions was evidenced in a mixed population of immune cells derived from mice spleens after incubation with polysaccharides by flow cytometry, MTT and Immunospot assays. These fractions, except F2, demonstrated selective immunostimulant activity, and the F1 fraction induced the most potent effect, closely followed by the F3 and F4 fractions. The in vivo mechanism of their action is associated with the activation of innate immunity and shapes the immune response to the Th1 type.
Název v anglickém jazyce
Immunologically active cell wall polysaccharides of green microalga Dictyosphaerium chlorelloides (Chlorellacea)
Popis výsledku anglicky
Dictyosphaerium chlorelloides is a green microalga from the Chlorella clade that produces highly viscous exocellular polysaccharides. The cell wall polysaccharides of this alga have not been studied in detail. In this article, water-soluble polysaccharides from D. chlorelloides biomass were extracted with hot water and purified by preparative chromatography. The composition, structural features and molecular masses of subsequently eluted fractions F1, F2, F3, F4 and F5 (minor) were determined. Three high-yield products F1, F3 and F4 consisted mainly of galactopyranosyl, 2-O-methyl-galactopyranosyl, rhamnopyranosyl and mannopyranosyl units at different proportions, while F2 was rich in glucose. Immunoactivity of these fractions was evidenced in a mixed population of immune cells derived from mice spleens after incubation with polysaccharides by flow cytometry, MTT and Immunospot assays. These fractions, except F2, demonstrated selective immunostimulant activity, and the F1 fraction induced the most potent effect, closely followed by the F3 and F4 fractions. The in vivo mechanism of their action is associated with the activation of innate immunity and shapes the immune response to the Th1 type.
Klasifikace
Druh
J<sub>SC</sub> - Článek v periodiku v databázi SCOPUS
CEP obor
—
OECD FORD obor
10606 - Microbiology
Návaznosti výsledku
Projekt
Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.
Návaznosti
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Ostatní
Rok uplatnění
2025
Kód důvěrnosti údajů
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Údaje specifické pro druh výsledku
Název periodika
Carbohydrate Polymers
ISSN
0144-8617
e-ISSN
1879-1344
Svazek periodika
353
Číslo periodika v rámci svazku
April 1
Stát vydavatele periodika
NL - Nizozemsko
Počet stran výsledku
16
Strana od-do
123242
Kód UT WoS článku
001422623800001
EID výsledku v databázi Scopus
2-s2.0-85214787864