Comparative lipidomics of snow algal blooms

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388971%3A_____%2F25%3A00618994" target="_blank" >RIV/61388971:_____/25:00618994 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/67985939:_____/25:00618994 RIV/00216208:11310/25:10500885

Výsledek na webu

<a href="https://doi.org/10.4490/algae.2025.40.2.11" target="_blank" >https://doi.org/10.4490/algae.2025.40.2.11</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.4490/algae.2025.40.2.11" target="_blank" >10.4490/algae.2025.40.2.11</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Comparative lipidomics of snow algal blooms

Popis výsledku v původním jazyce

Snow algae are excellent models for elucidating adaptations to abiotic stresses that characterize their extreme habitat. In cold-adapted microorganisms, changes in lipid composition represent an important strategy that enables survival at low temperatures. However, our knowledge in this field remains fragmentary. Using shotgun lipidomics, we identified 303 lipid species in field samples of snow algal blooms originating from 14 sites across a wide altitudinal gradient in mountains of Central Europe. Red, orange, and green snow blooms caused by vegetative cells or cysts of species from the genera Sanguina, Chloromonas, and Chlainomonas (Chlamydomonadales, Chlorophyta) were sampled. The analysis of total lipids using hydrophilic interaction chromatography showed that the samples were dominated by sphingolipids and triacylglycerols, forming 39.9-50.5% and 21.9-31.8% out of total lipids, respectively. Significant variability in lipid composition was revealed, reflecting differences in life cycle stage or metabolic setting and species composition among blooms of different color. Vegetative cells were characterized by a higher proportion of phospholipids (mean 18.0% vs. 13.5%. in orange and 14.4% in red cysts) and glycolipids (mean 17.3% vs. 13.2% and 10.3%), whereas triacylglycerols were less represented compared to the other two groups (mean 22.3% vs. 30.2% and 28.3%). This pattern was in line with the assumed physiological difference between the two main stages of the life cycle in chlamydomonadacean snow algae. A higher degree of unsaturation of phospholipid species in algal cells causing red blooms suggested a better adaptation of membranes to low temperatures compared to green and orange snow blooms.

Název v anglickém jazyce

Comparative lipidomics of snow algal blooms

Popis výsledku anglicky

Snow algae are excellent models for elucidating adaptations to abiotic stresses that characterize their extreme habitat. In cold-adapted microorganisms, changes in lipid composition represent an important strategy that enables survival at low temperatures. However, our knowledge in this field remains fragmentary. Using shotgun lipidomics, we identified 303 lipid species in field samples of snow algal blooms originating from 14 sites across a wide altitudinal gradient in mountains of Central Europe. Red, orange, and green snow blooms caused by vegetative cells or cysts of species from the genera Sanguina, Chloromonas, and Chlainomonas (Chlamydomonadales, Chlorophyta) were sampled. The analysis of total lipids using hydrophilic interaction chromatography showed that the samples were dominated by sphingolipids and triacylglycerols, forming 39.9-50.5% and 21.9-31.8% out of total lipids, respectively. Significant variability in lipid composition was revealed, reflecting differences in life cycle stage or metabolic setting and species composition among blooms of different color. Vegetative cells were characterized by a higher proportion of phospholipids (mean 18.0% vs. 13.5%. in orange and 14.4% in red cysts) and glycolipids (mean 17.3% vs. 13.2% and 10.3%), whereas triacylglycerols were less represented compared to the other two groups (mean 22.3% vs. 30.2% and 28.3%). This pattern was in line with the assumed physiological difference between the two main stages of the life cycle in chlamydomonadacean snow algae. A higher degree of unsaturation of phospholipid species in algal cells causing red blooms suggested a better adaptation of membranes to low temperatures compared to green and orange snow blooms.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10606 - Microbiology

Projekt

<a href="/cs/project/GA24-10019S" target="_blank" >GA24-10019S: Sněžné řasy v době klimatické změny: odhalování diverzity a sezónní dynamiky k porozumění současného a budoucího rozšíření</a><br>

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2025

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Algae

ISSN

1226-2617

e-ISSN

2093-0860

Svazek periodika

40

Číslo periodika v rámci svazku

1

Stát vydavatele periodika

KR - Korejská republika

Počet stran výsledku

13

Strana od-do

67-79

Kód UT WoS článku

001459242900005

EID výsledku v databázi Scopus

2-s2.0-105001438394