Recovery of β-Carotene from Microalga Dunaliella sp. by HPCCC

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388971%3A_____%2F25%3A00637066" target="_blank" >RIV/61388971:_____/25:00637066 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/60076658:12310/25:43909935

Výsledek na webu

<a href="https://www.mdpi.com/2227-9717/13/6/1812" target="_blank" >https://www.mdpi.com/2227-9717/13/6/1812</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.3390/pr13061812" target="_blank" >10.3390/pr13061812</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Recovery of β-Carotene from Microalga Dunaliella sp. by HPCCC

Popis výsledku v původním jazyce

beta-carotene, a high-value carotenoid widely used in the food, pharmaceutical, and cosmetics industries, is naturally synthesized by the microalga Dunaliella sp. However, the efficient extraction and purification of beta-carotene from microalgae biomass remain a technical challenge. This study presents the development of a scalable and efficient isolation method employing high-performance countercurrent chromatography (HPCCC) to recover beta-carotene from Dunaliella sp. The separation process was optimized by integrating two elution strategies (reverse phase and extrusion) using a biphasic solvent system of n-heptane and methanol (1:1, v/v). The upper phase served as the stationary phase, while the lower phase was used as the mobile phase. Two consecutive injections of 800 mg of microalgal extract each resulted in the isolation of 225.4 mg of beta-carotene with a purity of 97% and a recovery of 98%. The developed HPCCC approach represents an efficient method for beta-carotene purification and serves as a promising model for future scale-up in microalgae-based production platforms.

Název v anglickém jazyce

Recovery of β-Carotene from Microalga Dunaliella sp. by HPCCC

Popis výsledku anglicky

beta-carotene, a high-value carotenoid widely used in the food, pharmaceutical, and cosmetics industries, is naturally synthesized by the microalga Dunaliella sp. However, the efficient extraction and purification of beta-carotene from microalgae biomass remain a technical challenge. This study presents the development of a scalable and efficient isolation method employing high-performance countercurrent chromatography (HPCCC) to recover beta-carotene from Dunaliella sp. The separation process was optimized by integrating two elution strategies (reverse phase and extrusion) using a biphasic solvent system of n-heptane and methanol (1:1, v/v). The upper phase served as the stationary phase, while the lower phase was used as the mobile phase. Two consecutive injections of 800 mg of microalgal extract each resulted in the isolation of 225.4 mg of beta-carotene with a purity of 97% and a recovery of 98%. The developed HPCCC approach represents an efficient method for beta-carotene purification and serves as a promising model for future scale-up in microalgae-based production platforms.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

20401 - Chemical engineering (plants, products)

Projekt

—

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2025

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Processes

ISSN

2227-9717

e-ISSN

2227-9717

Svazek periodika

13

Číslo periodika v rámci svazku

6

Stát vydavatele periodika

CH - Švýcarská konfederace

Počet stran výsledku

15

Strana od-do

1812

Kód UT WoS článku

001515002300001

EID výsledku v databázi Scopus

2-s2.0-105009298342