Spatial structure of yeast biofilms and the role of cell adhesion across different media

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388971%3A_____%2F25%3A00640234" target="_blank" >RIV/61388971:_____/25:00640234 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/00216208:11310/25:10501740

Výsledek na webu

<a href="https://www.sciencedirect.com/science/article/pii/S2590207525000541?via%3Dihub" target="_blank" >https://www.sciencedirect.com/science/article/pii/S2590207525000541?via%3Dihub</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.bioflm.2025.100306" target="_blank" >10.1016/j.bioflm.2025.100306</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Spatial structure of yeast biofilms and the role of cell adhesion across different media

Popis výsledku v původním jazyce

The ability of yeast cells to adhere to solid surfaces or even penetrate semi-solid surfaces and form multicellular biofilms are critical factors in infection. This study examines the relationship between cell adhesion capability and the ability to create spatially organized biofilms in selected Saccharomyces cerevisiae strains, including clinical isolates, and five Candida species (C. albicans, C. glabrata, C. krusei, C. parapsilosis, and C. tropicalis). We assessed cell adhesion to polystyrene surface in four media varying in source of carbon and other nutrients. Using microscopy of vertical cell arrangement profiles within yeast populations grown at the solid-liquid interface, we evaluated their internal organization to determine whether the populations exhibit typical biofilm characteristics, such as the spatial organization of distinct cell types. Results indicate that well adherent S. cerevisiae strains form spatial biofilms with typical internal organization, highlighting strain-specific responses to media composition and supporting the use of natural S. cerevisiae strains for biofilm research. Among Candida species, biofilm formation did not consistently align with adhesion efficiency to plastic, while C. albicans and C. krusei formed spatially structured biofilms on media where they adhered well, C. tropicalis and C. glabrata exhibited efficient adhesion without biofilm structuring. Interestingly, C. parapsilosis formed a structured biofilm despite minimal adhesion. These findings emphasize the role of media composition, particularly components of yeast extract and defined medium for mammalian cell growth RPMI, which differentially impacted adhesion and

Název v anglickém jazyce

Spatial structure of yeast biofilms and the role of cell adhesion across different media

Popis výsledku anglicky

The ability of yeast cells to adhere to solid surfaces or even penetrate semi-solid surfaces and form multicellular biofilms are critical factors in infection. This study examines the relationship between cell adhesion capability and the ability to create spatially organized biofilms in selected Saccharomyces cerevisiae strains, including clinical isolates, and five Candida species (C. albicans, C. glabrata, C. krusei, C. parapsilosis, and C. tropicalis). We assessed cell adhesion to polystyrene surface in four media varying in source of carbon and other nutrients. Using microscopy of vertical cell arrangement profiles within yeast populations grown at the solid-liquid interface, we evaluated their internal organization to determine whether the populations exhibit typical biofilm characteristics, such as the spatial organization of distinct cell types. Results indicate that well adherent S. cerevisiae strains form spatial biofilms with typical internal organization, highlighting strain-specific responses to media composition and supporting the use of natural S. cerevisiae strains for biofilm research. Among Candida species, biofilm formation did not consistently align with adhesion efficiency to plastic, while C. albicans and C. krusei formed spatially structured biofilms on media where they adhered well, C. tropicalis and C. glabrata exhibited efficient adhesion without biofilm structuring. Interestingly, C. parapsilosis formed a structured biofilm despite minimal adhesion. These findings emphasize the role of media composition, particularly components of yeast extract and defined medium for mammalian cell growth RPMI, which differentially impacted adhesion and

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10606 - Microbiology

Projekt

<a href="/cs/project/EH22_008%2F0004573" target="_blank" >EH22_008/0004573: Průlomové laserové technologie pro chytrou výrobu, vesmírné a biotechnologické aplikace</a><br>

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2025

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Biofilm

ISSN

2590-2075

e-ISSN

2590-2075

Svazek periodika

10

Číslo periodika v rámci svazku

December 2025

Stát vydavatele periodika

NL - Nizozemsko

Počet stran výsledku

12

Strana od-do

100306

Kód UT WoS článku

001578007200001

EID výsledku v databázi Scopus

2-s2.0-105010869478