Is non-buffered DMEM solution a suitable medium for in vitro bioactivity tests?
Identifikátory výsledku
Kód výsledku v IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388980%3A_____%2F14%3A00436223" target="_blank" >RIV/61388980:_____/14:00436223 - isvavai.cz</a>
Výsledek na webu
<a href="http://dx.doi.org/10.1039/c4tb00187g" target="_blank" >http://dx.doi.org/10.1039/c4tb00187g</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1039/c4tb00187g" target="_blank" >10.1039/c4tb00187g</a>
Alternativní jazyky
Jazyk výsledku
angličtina
Název v původním jazyce
Is non-buffered DMEM solution a suitable medium for in vitro bioactivity tests?
Popis výsledku v původním jazyce
Several laboratories had tested bioactivity of the materials in commercially available solution DMEM (Dulbecco's Modified Eagle's Medium) that is normally used for cultivation of cell cultures. The objective of this work was to find out whether it is possible to replace TRIS-buffered SBF currently used for bioactivity tests with the non-buffered DMEM solution. To understand the role of the organic part of the DMEM solution in the process of crystallization, we have prepared non-buffered solution simulating only its inorganic part (identified as I-solution). It was found that under static-dynamic test conditions calcite (CaCO3) and the amorphous phase of calcium phosphate (ACP) formed on the surface of the glass-ceramic (45S5 bioactive glass based) scaffold exposed to both solutions. Additionally, halite (NaCl) formed at the beginning of exposure to DMEM. Hydroxyapatite phase was not detected on the surface in either non-buffered solution. Organic components contained in the DMEM soluti
Název v anglickém jazyce
Is non-buffered DMEM solution a suitable medium for in vitro bioactivity tests?
Popis výsledku anglicky
Several laboratories had tested bioactivity of the materials in commercially available solution DMEM (Dulbecco's Modified Eagle's Medium) that is normally used for cultivation of cell cultures. The objective of this work was to find out whether it is possible to replace TRIS-buffered SBF currently used for bioactivity tests with the non-buffered DMEM solution. To understand the role of the organic part of the DMEM solution in the process of crystallization, we have prepared non-buffered solution simulating only its inorganic part (identified as I-solution). It was found that under static-dynamic test conditions calcite (CaCO3) and the amorphous phase of calcium phosphate (ACP) formed on the surface of the glass-ceramic (45S5 bioactive glass based) scaffold exposed to both solutions. Additionally, halite (NaCl) formed at the beginning of exposure to DMEM. Hydroxyapatite phase was not detected on the surface in either non-buffered solution. Organic components contained in the DMEM soluti
Klasifikace
Druh
J<sub>x</sub> - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)
CEP obor
CA - Anorganická chemie
OECD FORD obor
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Návaznosti výsledku
Projekt
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Návaznosti
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Ostatní
Rok uplatnění
2014
Kód důvěrnosti údajů
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Údaje specifické pro druh výsledku
Název periodika
Journal of Materials Chemistry B
ISSN
2050-750X
e-ISSN
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Svazek periodika
2
Číslo periodika v rámci svazku
31
Stát vydavatele periodika
GB - Spojené království Velké Británie a Severního Irska
Počet stran výsledku
9
Strana od-do
5068-5076
Kód UT WoS článku
000339861000013
EID výsledku v databázi Scopus
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