Graphene oxide normal (GO + Mn2+) and ultrapure: Short‐term impact on selected antioxidant stress markers and cytokines in NHDF and A549 cell lines

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388980%3A_____%2F21%3A00542566" target="_blank" >RIV/61388980:_____/21:00542566 - isvavai.cz</a>

Výsledek na webu

<a href="http://hdl.handle.net/11104/0319952" target="_blank" >http://hdl.handle.net/11104/0319952</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.3390/antiox10050765" target="_blank" >10.3390/antiox10050765</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Graphene oxide normal (GO + Mn2+) and ultrapure: Short‐term impact on selected antioxidant stress markers and cytokines in NHDF and A549 cell lines

Popis výsledku v původním jazyce

Since biological applications and toxicity of graphene‐based materials are structure dependent, studying their interactions with the biological systems is very timely and important. We studied short‐term (1, 24, and 48 h) effects of ultrapure (GO) and Mn2+ ‐contaminated (GOS) graphene oxide on normal human dermal fibroblasts (NHDF) and adenocarcinomic human alveolar basal epithelial cells (A549) using selected oxidative stress markers and cytokines: glutathione reductase (GR) and catalase (CAT) activity, total antioxidative capacity (TAC), and malondialdehyde (MDA) concentration, levels of vascular endothelial growing factor (VEGF), tumor necrosis factoralpha (TNF‐α), platelet‐derived growth factor‐BB (PDGF‐BB), and eotaxin. GOS induced higher levels of oxidative stress, measured with CAT activity, TAC, and MDA concentration than GO in both cell lines when compared to control cells. GR activity decreased in time in NHDF cells but increased in A549 cells. The levels of cytokines were related to the exposure time and graphene oxide type in both analyzed cell lines and their levels comparably increased over time. We observed higher TNF‐ α levels in NHDF and higher levels of VEGF and eotaxin in the A549 cell line. Both types of cells showed similar susceptibility to GO and GOS. We concluded that the short‐time exposure to GOS induced the stronger response of oxidative stress markers without collapsing the antioxidative systems of analysed cells. Increased levels of inflammatory cytokines after GO and GOS exposure were similar both in NHDF and A549 cells.

Název v anglickém jazyce

Graphene oxide normal (GO + Mn2+) and ultrapure: Short‐term impact on selected antioxidant stress markers and cytokines in NHDF and A549 cell lines

Popis výsledku anglicky

Since biological applications and toxicity of graphene‐based materials are structure dependent, studying their interactions with the biological systems is very timely and important. We studied short‐term (1, 24, and 48 h) effects of ultrapure (GO) and Mn2+ ‐contaminated (GOS) graphene oxide on normal human dermal fibroblasts (NHDF) and adenocarcinomic human alveolar basal epithelial cells (A549) using selected oxidative stress markers and cytokines: glutathione reductase (GR) and catalase (CAT) activity, total antioxidative capacity (TAC), and malondialdehyde (MDA) concentration, levels of vascular endothelial growing factor (VEGF), tumor necrosis factoralpha (TNF‐α), platelet‐derived growth factor‐BB (PDGF‐BB), and eotaxin. GOS induced higher levels of oxidative stress, measured with CAT activity, TAC, and MDA concentration than GO in both cell lines when compared to control cells. GR activity decreased in time in NHDF cells but increased in A549 cells. The levels of cytokines were related to the exposure time and graphene oxide type in both analyzed cell lines and their levels comparably increased over time. We observed higher TNF‐ α levels in NHDF and higher levels of VEGF and eotaxin in the A549 cell line. Both types of cells showed similar susceptibility to GO and GOS. We concluded that the short‐time exposure to GOS induced the stronger response of oxidative stress markers without collapsing the antioxidative systems of analysed cells. Increased levels of inflammatory cytokines after GO and GOS exposure were similar both in NHDF and A549 cells.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10402 - Inorganic and nuclear chemistry

Projekt

—

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2021

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Antioxidants

ISSN

2076-3921

e-ISSN

2076-3921

Svazek periodika

10

Číslo periodika v rámci svazku

5

Stát vydavatele periodika

CH - Švýcarská konfederace

Počet stran výsledku

18

Strana od-do

765

Kód UT WoS článku

000653369000001

EID výsledku v databázi Scopus

2-s2.0-85105525541