The role of (HCO3)− ions in SBF on the interaction with bioactive glass-ceramic scaffold

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388980%3A_____%2F23%3A00568299" target="_blank" >RIV/61388980:_____/23:00568299 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/46747885:24620/23:00012078

Výsledek na webu

<a href="https://doi.org/10.1016/j.mtchem.2022.101367" target="_blank" >https://doi.org/10.1016/j.mtchem.2022.101367</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.mtchem.2022.101367" target="_blank" >10.1016/j.mtchem.2022.101367</a>

Jazyk výsledku

angličtina

Název v původním jazyce

The role of (HCO3)− ions in SBF on the interaction with bioactive glass-ceramic scaffold

Popis výsledku v původním jazyce

Simulated Body Fluid (SBF) has concentrations of inorganic elements nearly equal to those of human blood plasma (BP). However, there are some differences between SBF and BP which can distort the results of in vitro bioactivity tests. Contrary to BP, SBF has a too low concentration of (HCO3)- ions (only 4 instead of 27 mmol·dm−3) and on the contrary a higher concentration of Cl− ions (introduced by the TRIS-HCl buffer). Indeed, the main difference between SBF and BP is the presence of the TRIS buffer in SBF which is used for neutral pH maintaining. The aim of this work was to study the effect of (HCO3)- ions in non-buffered SBF on the solubility of a bioactive glass-ceramic material and the formation of a new phase on the sample surface. A highly reactive glass-ceramic scaffold derived from 45S5 bioactive glass was tested under static-dynamic conditions using SBF solution with different (HCO3)- ion concentrations (4, 20 and 27 mmol·dm−3). The results were compared with data obtained using standardized buffered SBF solution. A non-buffered SBF solution with a concentration of (HCO3)- ions closer to the real concentration in BP was shown to have the potential to replace the standardized SBF solution since the ISO 23317:2014 using SBF can give false-positive results of in vitro bioactivity tests.

Název v anglickém jazyce

The role of (HCO3)− ions in SBF on the interaction with bioactive glass-ceramic scaffold

Popis výsledku anglicky

Simulated Body Fluid (SBF) has concentrations of inorganic elements nearly equal to those of human blood plasma (BP). However, there are some differences between SBF and BP which can distort the results of in vitro bioactivity tests. Contrary to BP, SBF has a too low concentration of (HCO3)- ions (only 4 instead of 27 mmol·dm−3) and on the contrary a higher concentration of Cl− ions (introduced by the TRIS-HCl buffer). Indeed, the main difference between SBF and BP is the presence of the TRIS buffer in SBF which is used for neutral pH maintaining. The aim of this work was to study the effect of (HCO3)- ions in non-buffered SBF on the solubility of a bioactive glass-ceramic material and the formation of a new phase on the sample surface. A highly reactive glass-ceramic scaffold derived from 45S5 bioactive glass was tested under static-dynamic conditions using SBF solution with different (HCO3)- ion concentrations (4, 20 and 27 mmol·dm−3). The results were compared with data obtained using standardized buffered SBF solution. A non-buffered SBF solution with a concentration of (HCO3)- ions closer to the real concentration in BP was shown to have the potential to replace the standardized SBF solution since the ISO 23317:2014 using SBF can give false-positive results of in vitro bioactivity tests.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10402 - Inorganic and nuclear chemistry

Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2023

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Materials Today Chemistry

ISSN

2468-5194

e-ISSN

2468-5194

Svazek periodika

28

Číslo periodika v rámci svazku

MAR

Stát vydavatele periodika

GB - Spojené království Velké Británie a Severního Irska

Počet stran výsledku

8

Strana od-do

101367

Kód UT WoS článku

000925565100001

EID výsledku v databázi Scopus

2-s2.0-85147004064