Radiolabeling of antibody for epitope of human carbonic anhydrase IX (IgG M75) by 61Cu and 64Cu and its biological testing

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61389005%3A_____%2F15%3A00450524" target="_blank" >RIV/61389005:_____/15:00450524 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/68378050:_____/15:00450524

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Radiolabeling of antibody for epitope of human carbonic anhydrase IX (IgG M75) by 61Cu and 64Cu and its biological testing

Popis výsledku v původním jazyce

Human carbonic anhydrase IX is a membrane enzyme that is significantly expressed in some types of cancer cells. The antibody IgG M75 was successfully conjugated with a new, copper-specific chelator ?phosphinate, the conjugation method was optimized and is well-reproducible. The conjugate was then labelled with two positron emitting copper radioisotopes, Cu-61 (3.333 h) and Cu-64 (12.701 h). Labelled conjugates were tested in vivo in mice with colorectal cancer xenografts. Copper radioisotopes were produced via Ni-nat(d,x) and Ni-64(p,n) reactions (Ni, 99.999 %, Goodfellow; Ni-64, 99.09 %, Isoflex). Specific activity of Cu-61 was 1 GBq/mg Cu and more, which was satisfactory for the labelling purposes. All reagents were analytical grade and were purifiedprior labelling with chelex-100 in sodium form, 100-200 mesh from metals surplus. Chelator ?phospinate was synthesized and characterized in our laboratories. Antibody IgG M75 was prepared by genetic engineering methods. Immunoconjugates

Název v anglickém jazyce

Radiolabeling of antibody for epitope of human carbonic anhydrase IX (IgG M75) by 61Cu and 64Cu and its biological testing

Popis výsledku anglicky

Human carbonic anhydrase IX is a membrane enzyme that is significantly expressed in some types of cancer cells. The antibody IgG M75 was successfully conjugated with a new, copper-specific chelator ?phosphinate, the conjugation method was optimized and is well-reproducible. The conjugate was then labelled with two positron emitting copper radioisotopes, Cu-61 (3.333 h) and Cu-64 (12.701 h). Labelled conjugates were tested in vivo in mice with colorectal cancer xenografts. Copper radioisotopes were produced via Ni-nat(d,x) and Ni-64(p,n) reactions (Ni, 99.999 %, Goodfellow; Ni-64, 99.09 %, Isoflex). Specific activity of Cu-61 was 1 GBq/mg Cu and more, which was satisfactory for the labelling purposes. All reagents were analytical grade and were purifiedprior labelling with chelex-100 in sodium form, 100-200 mesh from metals surplus. Chelator ?phospinate was synthesized and characterized in our laboratories. Antibody IgG M75 was prepared by genetic engineering methods. Immunoconjugates

Druh

O - Ostatní výsledky

CEP obor

BG - Jaderná, atomová a molekulová fyzika, urychlovače

OECD FORD obor

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Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2015

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů