Length computation of irradiated plasmid DNA molecules

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61389005%3A_____%2F18%3A00499497" target="_blank" >RIV/61389005:_____/18:00499497 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/68378271:_____/18:00499497

Výsledek na webu

<a href="http://dx.doi.org/10.1116/1.5050502" target="_blank" >http://dx.doi.org/10.1116/1.5050502</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1116/1.5050502" target="_blank" >10.1116/1.5050502</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Length computation of irradiated plasmid DNA molecules

Popis výsledku v původním jazyce

Compromised detection of short DNA fragments can result in underestimation of radiation-induced clustered DNA damage. The fragments can be detected with atomic force microscopy (AFM), followed by image analysis to compute the length of plasmid molecules. Plasmid molecules imaged with AFM are represented by open or closed curves, possibly with crossings. For the analysis of such objects, a dedicated algorithm was developed, and its usability was demonstrated on the AFM images of plasmid pBR322 irradiated with Co-60 gamma rays. The analysis of the set of the acquired AFM images revealed the presence of DNA fragments with lengths shorter than 300 base pairs that would have been neglected by a conventional detection method.

Název v anglickém jazyce

Length computation of irradiated plasmid DNA molecules

Popis výsledku anglicky

Compromised detection of short DNA fragments can result in underestimation of radiation-induced clustered DNA damage. The fragments can be detected with atomic force microscopy (AFM), followed by image analysis to compute the length of plasmid molecules. Plasmid molecules imaged with AFM are represented by open or closed curves, possibly with crossings. For the analysis of such objects, a dedicated algorithm was developed, and its usability was demonstrated on the AFM images of plasmid pBR322 irradiated with Co-60 gamma rays. The analysis of the set of the acquired AFM images revealed the presence of DNA fragments with lengths shorter than 300 base pairs that would have been neglected by a conventional detection method.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

20903 - Bioproducts (products that are manufactured using biological material as feedstock) biomaterials, bioplastics, biofuels, bioderived bulk and fine chemicals, bio-derived novel materials

Projekt

<a href="/cs/project/GJ17-03403Y" target="_blank" >GJ17-03403Y: Souvislost mezi strukturou stopy záření a komplexním DNA poškozením</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2018

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Biointerphases

ISSN

1934-8630

e-ISSN

—

Svazek periodika

13

Číslo periodika v rámci svazku

6

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

7

Strana od-do

—

Kód UT WoS článku

000454614500001

EID výsledku v databázi Scopus

2-s2.0-85059419374