Effect of elevated temperature and hydrocortisone addition on the proliferation of fibroblasts

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61389005%3A_____%2F24%3A00586608" target="_blank" >RIV/61389005:_____/24:00586608 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/00216208:11110/24:10482902 RIV/00216208:11310/24:10482902

Výsledek na webu

<a href="https://doi.org/10.1007/s00418-024-02295-9" target="_blank" >https://doi.org/10.1007/s00418-024-02295-9</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1007/s00418-024-02295-9" target="_blank" >10.1007/s00418-024-02295-9</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Effect of elevated temperature and hydrocortisone addition on the proliferation of fibroblasts

Popis výsledku v původním jazyce

Hyperthermia along with hydrocortisone (HC) are proven teratogens that can negatively influence embryo development during early pregnancy. Proliferation of cells is one of the main developmental processes during the early embryogenesis. This study was focused on testing the effect of elevated temperature and HC addition on proliferation of cells in in vitro cultures. The V79-4 cell line was treated with HC and cultured in vitro at 37 degrees C or 39 degrees C, respectively. To reveal the effect of both factors, the proliferation of cells cultured under different conditions was evaluated using various approaches (colony formation assay, generation of growth curves, computation of doubling times, and mitotic index estimation). Our results indicate that a short-term exposure to elevated temperature slightly stimulates and a long-term exposure suppresses cell proliferation. However, HC (0.1 mg/ml) acts as a stimulator of cell proliferation. Interestingly, the interaction of HC and long-term elevated temperature (39 degrees C) exposure results in at least partial compensation of the negative impact of elevated temperature by HC addition and in higher proliferation if compared with cells cultured at 39 degrees C without addition of HC.

Název v anglickém jazyce

Effect of elevated temperature and hydrocortisone addition on the proliferation of fibroblasts

Popis výsledku anglicky

Hyperthermia along with hydrocortisone (HC) are proven teratogens that can negatively influence embryo development during early pregnancy. Proliferation of cells is one of the main developmental processes during the early embryogenesis. This study was focused on testing the effect of elevated temperature and HC addition on proliferation of cells in in vitro cultures. The V79-4 cell line was treated with HC and cultured in vitro at 37 degrees C or 39 degrees C, respectively. To reveal the effect of both factors, the proliferation of cells cultured under different conditions was evaluated using various approaches (colony formation assay, generation of growth curves, computation of doubling times, and mitotic index estimation). Our results indicate that a short-term exposure to elevated temperature slightly stimulates and a long-term exposure suppresses cell proliferation. However, HC (0.1 mg/ml) acts as a stimulator of cell proliferation. Interestingly, the interaction of HC and long-term elevated temperature (39 degrees C) exposure results in at least partial compensation of the negative impact of elevated temperature by HC addition and in higher proliferation if compared with cells cultured at 39 degrees C without addition of HC.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10610 - Biophysics

Projekt

<a href="/cs/project/EF16_019%2F0000728" target="_blank" >EF16_019/0000728: Výzkum ultrastopových izotopů a jejich využití v sociálních a environmentálních vědách urychlovačovou hmotnostní spektrometrií</a><br>

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2024

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Histochemistry and Cell Biology

ISSN

0948-6143

e-ISSN

1432-119X

Svazek periodika

162

Číslo periodika v rámci svazku

3

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

14

Strana od-do

231-244

Kód UT WoS článku

001232188100001

EID výsledku v databázi Scopus

2-s2.0-85194529873