Quantification in MALDI-TOF mass spectrometry of modified polymers

Kód výsledku v IS VaVaI

RIV/61389013:_____/11:00359037 - isvavai.cz

Výsledek na webu

http://dx.doi.org/10.1016/j.aca.2011.03.019

DOI - Digital Object Identifier

10.1016/j.aca.2011.03.019

Jazyk výsledku

angličtina

Název v původním jazyce

Quantification in MALDI-TOF mass spectrometry of modified polymers

Popis výsledku v původním jazyce

MALDI-TOF MS quantification is hampered by the poor reproducibility and molecular-mass and compositional discrimination. The use of an internal standard increases reproducibility and allows a calibration curve to be constructed. With synthetic polymers,the method requires a polymer of the same molecular mass distribution and composition as the analyte. When the content of the precursor polymer in a modified polymer is sought, the pure precursor is usually available and the modified polymer can serve asan internal standard. The method has to be combined with that of standard addition and the precursor polymer added directly to the sample. The experiments with simulated modified polymers revealed the signal-intensity ratio not proportional to the concentrations ratio. Thus, the basic assumption of the standard-addition method is not met. Therefore, the multi-point combined internal-standard standard-addition method based on the power-type calibration curve was developed and verified.

Název v anglickém jazyce

Quantification in MALDI-TOF mass spectrometry of modified polymers

Popis výsledku anglicky

MALDI-TOF MS quantification is hampered by the poor reproducibility and molecular-mass and compositional discrimination. The use of an internal standard increases reproducibility and allows a calibration curve to be constructed. With synthetic polymers,the method requires a polymer of the same molecular mass distribution and composition as the analyte. When the content of the precursor polymer in a modified polymer is sought, the pure precursor is usually available and the modified polymer can serve asan internal standard. The method has to be combined with that of standard addition and the precursor polymer added directly to the sample. The experiments with simulated modified polymers revealed the signal-intensity ratio not proportional to the concentrations ratio. Thus, the basic assumption of the standard-addition method is not met. Therefore, the multi-point combined internal-standard standard-addition method based on the power-type calibration curve was developed and verified.

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

CC - Organická chemie

OECD FORD obor

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Projekt

GA203/08/0543: Hybridní makromolekulární systémy na bázi hydrofilních polymerů a peptidů reagujících na vnější podněty

Návaznosti

Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2011

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Analytica Chimica Acta

ISSN

0003-2670

e-ISSN

—

Svazek periodika

693

Číslo periodika v rámci svazku

1

Stát vydavatele periodika

NL - Nizozemsko

Počet stran výsledku

7

Strana od-do

82-88

Kód UT WoS článku

000290886800010

EID výsledku v databázi Scopus

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